We narrowed to 6,371 results for: siae
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Plasmid#83235PurposepGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeastDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR
Plasmid#123346PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCerulean3-Cerulean3-FLARE-CKAR
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A
Plasmid#159167PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A
TagsSV40 NLSExpressionYeastMutationMutated Met 7 of the cyt b562 module of nuc-yHS1 …PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-GFP-FLARE-AKAR
Plasmid#123332PurposeGreen-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertGFP-GFP-FLARE-AKAR
Tags6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …ExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED9
Plasmid#15407DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dGZmxh
Plasmid#44552DepositorInsertsPTETREG promoter
yEGFP::ZeoR
UseSynthetic Biology; Expression regulator/reporterExpressionYeastPromoterPTETREGAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
ExpressionBacterial and YeastPromoterpADH1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-H840A
Plasmid#113024PurposeExpresses GCN4-H840A in mammalian cellsDepositorInsertGCN4-H840A (GCN4 Budding Yeast, S. pyogenes)
ExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable SinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-Venus-FLARE-AKAR
Plasmid#123331PurposeYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertVenus-Venus-FLARE-AKAR
Tags6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…ExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV
Plasmid#123342PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.DepositorInsertCerulean3-Cerulean3-FLARE-EKAR-EV
Tags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-H840A-GCN4
Plasmid#113027PurposeExpresses H840A-GCN4 in mammalian cellsDepositorInsertH840A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
ExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-EKAR-EV
Plasmid#123341PurposeRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.DepositorInsertmCherry-mCherry-FLARE-EKAR-EV
Tags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
myc-Med4 pBacPAK8
Plasmid#15364DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y
Plasmid#60393PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)DepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-MED9
Plasmid#15408DepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med9 pBacPAK8
Plasmid#15363DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-delta848-918
Plasmid#238313PurposeScVip1-536-1107-delta848-918 protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-delta848-918Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-R547A-H548A-R551A
Plasmid#238317PurposeScVip1-536-1107-R547A-H548A-R551A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-R547A-H548A-R551AAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-K554A-K556A-K644A
Plasmid#238328PurposeScVip1-536-1107-K554A-K556A-K644A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-K554A-K556A-K644AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-P553V-G646V-G647A
Plasmid#238322PurposeScVip1-536-1107-P553V-G646V-G647A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-P553V-G646V-G647AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-K817S-D819A-S821A
Plasmid#238326PurposeScVip1-536-1107-K817S-D819A-S821A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-K817S-D819A-S821AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED solvvol
Plasmid#232958PurposeGalactose iduced expression of Gcn4 LVtoED solvvol in yeastDepositorInsertGcn4 ILVtoED solvvol
TagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-CDA1
Plasmid#229534PurposepMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsCas3
Cytidine deaminase
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(25Q)-c_myc
Plasmid#225228PurposeYeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.DepositorInsertsAga2
Htt (25Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(46Q)-c_myc
Plasmid#225229PurposeYeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.DepositorInsertsAga2
Htt (46Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only