164,577 results

1. pT3-EF1aH NICD1

   Plasmid

   #86500

   Purpose

   Mammalian expression of Myc tagged NICD1

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain only (AA 1753-2531)

   Promoter

   EF1a

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-GFP-APEX2-NES_pLX208

   Plasmid

   #202018

   Purpose

   expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-GFP-APEX2-NES
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSems-leader-mXFPm-IFNGR1(18-489)

   Plasmid

   #192786

   Purpose

   Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNGR1 (IFNGR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence and mXFPm

   Expression

   Mammalian

   Mutation

   mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

   Plasmid

   #232879

   Purpose

   Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   5-Hydroxytryptamine Receptor 6 (HTR6 Human)
   

   Use

   Lentiviral

   Tags

   RGECO1.1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-MCS-ASC-GFP

   Plasmid

   #73957

   Purpose

   Express human ASC with a C-terminal EGFP tag in mammalian cells

   Depositor

   Christian Stehlik

   Article

   de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBRC_double_attB_GFP_donor

   Plasmid

   #169692

   Purpose

   cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion)

   Depositor

   John Wang

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac-TetO-En1-t2a-Foxa2-hygro

   Plasmid

   #176483

   Purpose

   TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV_IRES_GFP_CD16a

   Plasmid

   #196189

   Purpose

   Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

   Depositor

   Ana Ruiz-Saenz

   Article

   Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

   Insert

   CD16a (FCGR3A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 158 to Valine

   Promoter

   LTR

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AIO-GFP

   Plasmid

   #74119

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CSII-IDR2-chimpTRIM5-FOS

    Plasmid

    #79065

    Purpose

    Express chimpanzee TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(chimpanzee)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. dPspCas13b-wtFTO

    Plasmid

    #223696

    Purpose

    Expressing fusion protein of dPspCas13b and wild type human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-wtFTO (FTO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid

    #216154

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-PS Intein

    Plasmid

    #242020

    Purpose

    AAV version of PS Intein palsmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein
    

    Use

    Adenoviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV GFAP CaBLAM

    Plasmid

    #244228

    Purpose

    Bioluminescent reporter for calcium signaling in astrocytes

    Depositor

    Ute Hochgeschwender

    Article

    Lambert et al bioRxiv. 2023 Jun 26:2023.06.25.546478.

    Inserts

    mNeonGreen
    CaBLAM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAD21-HaloTag Vector

    Plasmid

    #238694

    Purpose

    Express RAD21-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    RAD21 (RAD21 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. IL13Ra1-HaloTag BiBRET Vector

    Plasmid

    #238673

    Purpose

    Express IL13Ra1-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IL13Ra1 (IL13RA1 Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFUW-TetO-NFATC2

    Plasmid

    #207191

    Purpose

    doxycycline-inducible expression of Human NFATC2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-NFATC2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CMV-Vcan-V3-HA

    Plasmid

    #238076

    Purpose

    Expresses Vcan V3 in mice heart tissues

    Depositor

    Yuxuan Guo

    Article

    Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

    Insert

    Vcan (Vcan Mouse)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP

    Plasmid

    #187072

    Purpose

    Mitochondria surface expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSIN4-BCAT1

    Plasmid

    #240021

    Purpose

    Expresses human BCAT1 fused to 3X Flag in mammalian cells

    Depositor

    Rui Su

    Insert

    BCAT1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HaloTag-hCHMP4B Vector

    Plasmid

    #236972

    Purpose

    Express HaloTag(R)-hCHMP4B Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    hCHMP4B (CHMP4B Human)
    

    Use

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0444

    Plasmid

    #143701

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFX (ZFX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSF18

    Plasmid

    #222593

    Purpose

    BB2 ZWF1 tet-on overexpression cassette

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    ZWF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    tet-on

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-iGlu3slow1

    Plasmid

    #226501

    Purpose

    Superslow-decay iGluSnFR3 variant 1 for population imaging

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 D25A
    

    Use

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    D25A

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-iGlu3slow2

    Plasmid

    #226502

    Purpose

    Superslow-decay iGluSnFR3 variant 2 for population imaging

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 D25R
    

    Use

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    D25R

    Promoter

    CMV

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lifeact-mTFP1

    Plasmid

    #218980

    Purpose

    In vivo visualization of actin cytoskeleton

    Depositor

    Amro Hamdoun

    Insert

    Lifeact-mTFP1
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CAG-ConVERGD-eGFP-W3SL

    Plasmid

    #218748

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AA184

    Plasmid

    #216047

    Purpose

    Fragmid fragment: (C' terminus) for prime editing; using PEmax

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    SV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AA063

    Plasmid

    #216020

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpRY (H840A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AA061

    Plasmid

    #216018

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-NG (H840A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AA058

    Plasmid

    #216040

    Purpose

    Fragmid fragment: (C' terminus) for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    MMLV-RT_v1.1; SV40NLS_v1.3
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AA183

    Plasmid

    #216025

    Purpose

    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (H840A)_v2.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AA062

    Plasmid

    #216019

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpG (H840A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AA060

    Plasmid

    #216017

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for prime editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (H840A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFlare9A-Plekha5E10 WT

    Plasmid

    #209576

    Purpose

    minigene construct for Plekha5 E10 alternative splicing

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Plekha5 (Plekha5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIPZ-NUDT18-C-FLAG-Puro

    Plasmid

    #195181

    Purpose

    Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT18 (NUDT18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF1074

    Plasmid

    #143753

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC4 (NFATC4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBetaActin-TUBB2a-mScarlet

    Plasmid

    #191324

    Purpose

    Expresses fluorescent labeled beta Tubulin to measure microtubule density

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mScarlet (Tubb2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge