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179,521 results

Showing: 7801 - 7840 of 179521 results

  1. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Publication
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131464-plasmid-map-sequence-id-261686

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  2. pCMV-Sport6-CD63-pHuji

    Plasmid
    #130902
    Purpose
    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD63-pHuji (CD63 Human, synthetic)
    Tags
    pHuji
    Expression
    Mammalian
    Mutation
    pHuji inserted between Gln-36 and Leu-37 in extra…
    Promoter
    CMV
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130902-plasmid-map-sequence-id-259045

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  3. HR180-LGR5-iCT

    Plasmid
    #129094
    Purpose
    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus
    Depositor
    Toshiro Sato
    Publication
    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    Use
    Homologous recombination donor plasmid
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129094-plasmid-map-sequence-id-252095

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  11. Lamin A-mRFP

    Plasmid
    #124268
    Purpose
    Wild-type Lamin A, C-terminally tagged with mRFP
    Depositor
    Eric Schirmer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124268-plasmid-map-sequence-id-241940

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  13. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Kevin McGowan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sec61 (SEC61B Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123285-plasmid-map-sequence-id-242690

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  17. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Publication
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

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  19. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    David Andrews
    Publication
    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:
    Insert
    activating transcription factor 4 (ATF4 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115970-plasmid-map-sequence-id-222082

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  21. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104492-plasmid-map-sequence-id-381181

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  29. pcDNA4 HisMax-V5-GFP-RRBP1

    Plasmid
    #92150
    Purpose
    Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.
    Depositor
    Alice Ting
    Publication
    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RRBP1 (RRBP1 Human)
    Tags
    Calreticulin signal peptide, EGFP (A207K mutation…
    Expression
    Mammalian
    Mutation
    In RRBP1: N951S and T1329L. E1279 is also missing.
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92150-plasmid-map-sequence-id-181297

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  30. pMH-SFB-BRCA2

    Plasmid
    #99395
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2
    Depositor
    Junjie Chen, Michael Huen
    Publication
    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRCA2 (BRCA2 Human)
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99395-plasmid-map-sequence-id-190616

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  31. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92396-plasmid-map-sequence-id-178234

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  32. pCMV-DeAct-SpvB

    Plasmid
    #89446
    Purpose
    Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89446-plasmid-map-sequence-id-177125

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  35. pMaster12

    Plasmid
    #58527
    Purpose
    Reprogram somatic cells to iPS cells
    Depositor
    Mario Capecchi
    Publication
    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    Expression
    Mammalian
    Available since
    Feb. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58527-plasmid-map-sequence-id-138420

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  38. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid
    #75013
    Purpose
    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.
    Depositor
    Martin Pule
    Publication
    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do
    Inserts
    murine GD3 synthase
    murine GD2 synthase
    Use
    Retroviral; Momlv retroviral vector
    Expression
    Mammalian
    Available since
    Sept. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75013-plasmid-map-sequence-id-141876

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  39. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  40. pKA-207I10

    Plasmid
    #79845
    Purpose
    Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
    Expression
    Mammalian
    Mutation
    RpPpsR2 cysteine 439 changed to serine
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79845-plasmid-map-sequence-id-149588

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Showing: 7801 - 7840 of 179521 results