We narrowed to 10,563 results for: CHL
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Plasmid#170081PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB1
Plasmid#162691PurposeExpresses DabAB1 inorganic carbon transport complexDepositorInsertDabAB1 inorganic carbon transport complex
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw
Plasmid#162473PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw
Plasmid#162478PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreAB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw
Plasmid#162476PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw
Plasmid#162475PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw
Plasmid#162472PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw
Plasmid#162470PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreA
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw
Plasmid#162477PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreC
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw
Plasmid#162471PurposeThis insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.DepositorInsertCreB
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95D293A
Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE669
Plasmid#153235PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE672
Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE473
Plasmid#153264PurposeM1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE500
Plasmid#153273PurposeM6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE502
Plasmid#153275PurposeM8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
C335
Plasmid#141125PurposeExpresses chimeric repressor (RbsR-L) under Cin promoterDepositorInsertsUseTagsssrA degradation tagExpressionBacterialMutationChimera: DNA binding domain of LacI and ligand bi…Promoterengineered cin promoter (responds to C14-HSL) and…Available sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP35-TD-HilA
Plasmid#153332PurposeTriggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertHA4-7c12 Tandem Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP92-TD-HilA
Plasmid#153331PurposeTriggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertSptP92-HA4-7c12 Tandem Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-3G124-HilA
Plasmid#153324PurposeShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vectorDepositorInsert3G124 DARPin but no expression
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MYMH 351
Plasmid#138058PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-10AG 11AC-GFP
UseTagsExpressionBacterialMutationAAA GAG GAG GCAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 336
Plasmid#138057PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC 11AC-GFP
UseTagsExpressionBacterialMutationGCA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 333
Plasmid#138056PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 11AC-GFP
UseTagsExpressionBacterialMutationGAA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 330
Plasmid#138055PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb- 2AC 11AC-GFP
UseTagsExpressionBacterialMutationACA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 327
Plasmid#138054PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC-GFP
UseTagsExpressionBacterialMutationGCA GAG GAG AAAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK110
Plasmid#110576PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP6 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK112
Plasmid#110577PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP12b Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK113
Plasmid#110578PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP32 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1a-T2-GpNLuc
Plasmid#135949PurposeExpresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-OgNLuc
Plasmid#135951PurposeExpresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_005
Plasmid#123992PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA homology arm-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_006
Plasmid#123993PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_007
Plasmid#123994PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_056
Plasmid#123921PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-hu6_20N_cr3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_026
Plasmid#123906PurposeEncodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthuman non targeting sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_028
Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_029
Plasmid#123909PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only