4,143 results

1. Cofilin-pmCherryC1

   Plasmid

   #27687

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Cofilin (Cfl1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGC-1 alpha promoter luciferase delta CRE

   Plasmid

   #8888

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the CREB bind…

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 deltaN47 Beta-catenin

   Plasmid

   #19287

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-47

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK603

   Plasmid

   #8831

   Depositor

   David Waugh

   Article

   Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

   Insert

   TEV protease, S219D mutant
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S219D mutant

   Promoter

   Available since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 811 pSG5L HA PTEN C124S

   Plasmid

   #10744

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C124S

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHA-PUMA

   Plasmid

   #16588

   Depositor

   Bert Vogelstein

   Article

   Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

   Insert

   PUMA (BBC3 Human)
   

   Use

   Tags

   2x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA cyclin D1 HA

   Plasmid

   #11181

   Depositor

   Bruce Zetter

   Article

   Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

   Insert

   cyclin D1 (CCND1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS424GFP-FYVE(EEA1)

    Plasmid

    #36096

    Depositor

    Scott Emr

    Article

    Burd et al Mol Cell. 1998 Jul;2(1):157-62.

    Insert

    FYVE(EEA1) domain (EEA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PRC1

    Available since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL SFFV d14GFP Donor

    Plasmid

    #31475

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted 14 amino acids on the c terminus of eGFP

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT/TO/FLAG/HA-DEST DICER

    Plasmid

    #19881

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEZYegfp

    Plasmid

    #18671

    Purpose

    pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe puro Snail

    Plasmid

    #23347

    Depositor

    Bob Weinberg

    Article

    Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

    Insert

    Snail (SNAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVPT-rtTR-KRAB-2SM2

    Plasmid

    #11652

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-HA GST RagA wt

    Plasmid

    #19298

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding A (RRAGA Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLOVE-GFP

    Plasmid

    #15949

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

    Insert

    Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cav1-mRFP

    Plasmid

    #14434

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge