4,483 results
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Plasmid#13378DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRanGAP (RANGAP1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6-DEST PINK1-V5 KD
Plasmid#13319DepositorInsertPINK1 (PINK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationKinase dead: K219A/D362A/D384AAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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1271 pBabe puroL Myr HA Akt2
Plasmid#9018DepositorAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRT043-CLYBL-DDdcas9-VPH
Plasmid#158091PurposeInducible expression of dCas9-VPH from the CLYBL locus for CRISPR activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLYBL-CAG-DDdCas9VPH-T2A-GFP
UseTALENExpressionMammalianPromoterCAGAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEx-ER-TurboID
Plasmid#160857PurposeCre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas serviceAAV8InsertER TurboID
UseAAVTagsHA and V5PromoterCAGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas serviceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts8xARE- minimal promoter - GFP
TagBFP
ExpressionMammalianPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Giantin
Plasmid#98880PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGiantin
TagsmNeonGreenExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
Tagsstrep tagExpressionBacterialPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV CD68-hM4D(Gi)-mCherry
Plasmid#75033PurposeExpression of hM4D(Gi) using the CD68 promoter.DepositorHas serviceAAV1 and AAV9InserthM4D(Gi)-mCherry
UseAAVExpressionMammalianPromoterCD68Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIBN (CIB1 Mustard Weed)
TagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BLM
Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBLM (BLM Human)
TagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRPromotermU6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationSRDX (X3) repressor fusion; NLS fusionAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPa
Plasmid#66978PurposeExpression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of proteinDepositorAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by