4,508 results
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Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PPRE-H2B-eGFP
Plasmid#84393PurposeFluorescent reporter for PPARgamma activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPARG response element (PPARG Human)
TagseGFPExpressionMammalianPromoterPPREAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
Tagsstrep tagExpressionBacterialPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
TagsNoneExpressionBacterialMutationNonePromoterheat-shock inducibleAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hLbCpf1(TYCV) (pY230)
Plasmid#89355PurposeExpresses humanized LbCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationG532R/K595RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIBN (CIB1 Mustard Weed)
TagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9
TagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB505
Plasmid#74847PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRMutationVP64 activator fusion; NLS fusionAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 Flag C/EBPa
Plasmid#66978PurposeExpression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of proteinDepositorAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL-MHCK7-GCaMP6
Plasmid#65042PurposeLentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7DepositorInsertGCaMP6
UseLentiviralTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianPromoterMHCK7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIN4-EF1a-ETV2-IRES-Puro
Plasmid#61061Purposelentiviral vector for ETV2DepositorAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-Neo
Plasmid#60525PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phDLX2-N174
Plasmid#60860Purpose2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoterDepositorAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST
Plasmid#49771PurposeLevel 1 hCas9 moduleDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert35Sp::hCas9
UseCRISPRAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
paavCAG-Jx-rev-post-mGRASP-2A-dTomato
Plasmid#34913PurposeJxON-postDepositorInsertpostsynaptic mGRASP
UseAAV and Cre/LoxPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by