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Kir2.1 K+ channel scFv [N112B/29]

Plasmid

#206770

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112B/29 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pM5MF529

Plasmid

#17686

Depositor

David Shalloway

Article

Lin et al Oncogene. 1995 Jan 19. 10(2):401-5.

Insert

c-src Y529F (Src Mouse)

Expression

Mammalian

Mutation

Tyr 529 changed to Phe .

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pVP16

Plasmid

#21016

Depositor

Corey Largman

Insert

MEIS1A (Meis1 Mouse)

Tags

VP16-AD

Expression

Mammalian

Mutation

VP16 activation domain fused to N-terminal

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Aequorea victoria, Synthetic, Mouse)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR223 R2pH-LAMP1-3xFLAG

Plasmid

#157941

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG, mCherry, and pHluorin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CACNA1E KI

Plasmid

#131481

Purpose

Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC Flag-mRAP1

Plasmid

#73135

Purpose

Expression of Flag-tagged mRAP1

Depositor

Titia de Lange, Agnel Sfeir

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLDPuro-MmnSR100N

Plasmid

#35171

Depositor

Benjamin Blencowe

Article

Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

Insert

Srrm4 (Srrm4 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha2 [N399/19R]

Plasmid

#177544

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha2 (Rat). Derived from hybridoma N399/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQUASp-mCD8GFP

Plasmid

#46163

Purpose

Expression of murine CD8GFP in insects

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

mCD8GFP

Expression

Insect

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-ATG16-C-18

Plasmid

#54991

Purpose

Localization: Membrane Receptor, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ATG16

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
61426-hU6-shmFmr1-EF1a-mCherry

Plasmid

#157859

Purpose

Expresses a shRNA targeting mouse Fmr1 gene

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Fmr1 (Fmr1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti GW V5 Eco/Dam mse LaminA

Plasmid

#182674

Purpose

Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

Depositor

Bas van Steensel

Article

Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

Insert

Lamin A (Lmna Mouse)

Use

Lentiviral

Tags

E. Coli Dam and V5

Expression

Mammalian

Promoter

HSP

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mPax3-3xHA

Plasmid

#27319

Depositor

Jonathan Epstein

Article

Epstein et al J Biol Chem. 1995 May 19. 270(20):11719-

Insert

Pax3 (Pax3 Mouse)

Tags

3X HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
GBSGFP in pBS-KS

Plasmid

#121974

Purpose

Gli activity reporter

Depositor

Hiroshi Sasaki

Article

Sasaki et al Development. 1997 Apr . 124(7):1313-22.

Insert

GBSGFP (Gnb5 Mouse)

Use

Cloning

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Utf1

Plasmid

#13369

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

undifferentiated embryonic cell transcription factor 1 (Utf1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM4-N-22

Plasmid

#54168

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM4

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMIG II SIN-SV40-mCherry-SHP1

Plasmid

#180799

Purpose

Expression of mouse SHP1 fused to N-terminal mCherry

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SHP1 (Nr0b2 Mouse)

Use

Retroviral

Promoter

SV40

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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