We narrowed to 7,181 results for: RAP

1. pCMV-HA-JMJD2A

   Plasmid

   #24180

   Purpose

   Mammalian expression of human JMJD2A with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2A (KDM4A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. C413-E45: attB4-hr5-ie1p>-attB1r

   Plasmid

   #162930

   Purpose

   Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV ie1 promoter with hr5 enhancer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-nuclear-EKAR4

   Plasmid

   #174439

   Purpose

   Nuclear-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   nuclear-EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN mCherry-OPTN(D474N)

   Plasmid

   #119683

   Purpose

   For mammalian expression of UBD mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D474N

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK0_062

   Plasmid

   #123951

   Purpose

   Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR DEST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CFP-GAI(1-172)

   Plasmid

   #37309

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   contains aa 1-172

   Promoter

   CMV

   Available since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pRDB_118

   Plasmid

   #216089

   Purpose

   Cas9 [Sp] CRISPRa targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

   Plasmid

   #223160

   Purpose

   Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   dGFP-P2A-NanoLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TB:5xNF- κβ_RE:MiniP

   Plasmid

   #133878

   Purpose

   5 copies of the NF-κβ DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL MND WASp

    Plasmid

    #36248

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MND

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH6HTC_BRD2-Halo

    Plasmid

    #175336

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK1289-AAV-dCjCas9

    Plasmid

    #223138

    Purpose

    AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_PGK_SNIPR_CD28 SNIPR

    Plasmid

    #188358

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

    Plasmid

    #188374

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL703

    Plasmid

    #140373

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-2BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDA_833

    Plasmid

    #216086

    Purpose

    Cas9 [Sp] knockout targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBMN iRFP670-OPTN(D474N)

    Plasmid

    #119693

    Purpose

    For mammalian expression of iRFP-tagged UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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