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Showing: 41 - 80 of 947 results

  1. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  4. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  12. pBAD33.1-DpnII

    Plasmid
    #127413
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Depositor
    Douglas Feldman
    Insert
    DpnII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    127413-plasmid-map-sequence-id-247669

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  13. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  14. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  15. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PN25
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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  17. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62818-plasmid-map-sequence-id-114423

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  18. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  19. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Depositor
    Jan-Henning Klusmann
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181981-plasmid-map-sequence-id-366611

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  23. pKB01_tagRFP

    Plasmid
    #69103
    Purpose
    Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.
    Depositor
    Jan-Willem Veening
    Article
    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:
    Insert
    tagRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69103-plasmid-map-sequence-id-125507

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  26. pTH1-SpSrtA

    Plasmid
    #64987
    Purpose
    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955
    Insert
    S. pyogenes sortase A
    Use
    Tags
    His6 tag, Mxe GyrA intein, and chitin-binding pro…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-81
    Promoter
    T7 promoter
    Available since
    June 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    64987-plasmid-map-sequence-id-109847

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  27. pCri-H6-TEV-GRAB

    Plasmid
    #128498
    Purpose
    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
    Depositor
    F Xavier Gomis-Ruth
    Article
    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.
    Insert
    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    Use
    Tags
    His tag (6x)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    128498-plasmid-map-sequence-id-251962

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  28. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  36. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133340-plasmid-map-sequence-id-269542

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Showing: 41 - 80 of 947 results