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Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHA_SBV_NSs
Plasmid#208938PurposeExpresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 47 bp at 5' end and 510 bp from 3…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Portal_prophage_Desulfurococcales_archaeon
Plasmid#188816PurposePortal protein from Desulfurococcales archaeon ex4484_217_2 prophageDepositorInsertPortal
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Terminase_prophage_Desulfurococcales_archaeon
Plasmid#188841PurposeLarge terminase from Desulfurococcales archaeon ex4484_217_2 prophageDepositorInsertTerminase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge_wR
Plasmid#139119Purposeexpress hnRNPA2 LC with no charged residues with R added backDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationremove all K, most DPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCHAC-mt-mKeima
Plasmid#72342Purposeretrovirus construct for stable expressing mt-mKeimaDepositorInsertmt-mKeima
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mChartreuse
Plasmid#219397PurposeConstitutive expression of mChartreuse in E. coliDepositorInsertmChartreuse
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-α-chain
Plasmid#133730PurposeExpresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsa-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (aa1-141 + stop…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human J-chain
Plasmid#158215PurposeExpress the human J-chainDepositorInsertJCHAIN (JCHAIN Human)
UseTags8×hisExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-β-chain
Plasmid#133731PurposeExpresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsb-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (startcodon + a…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-1
Plasmid#90325PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-2
Plasmid#90326PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-J chain
Plasmid#145146PurposeMammalian expression plasmid for J chain of polymeric antibody classesDepositorInsertimmunoglobulin J chain (JCHAIN Human)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MXS_chaining vector
Plasmid#62394PurposeEmpty BackboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CHAMP1
Plasmid#159892PurposeExpresses CHAMP1 (CAMP) in mammalian cellsDepositorInsertCHAMP1 (CHAMP1 Human)
UseTagspEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTMB352_ZFcharm_Kv1_split-inteins
Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-MFSD12-V5
Plasmid#149508PurposeStable expression of human MFSD12 with recoded nucleotide sequence.DepositorInsertMFSD12 (MFSD12 Human)
UseLentiviralTagsV5ExpressionMutationCodon Optimized SequencePromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRG36-ChAT
Plasmid#99069PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)DepositorInsertChAT (choline acetyltransferase) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase_PCi_PC-σ
Plasmid#214040PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceumDepositorInsertSAVED-CHAT-Pcaspase-Pci-PC-σ
UseTagsExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDZ2FE_Champ1_1-812
Plasmid#105675PurposeExpresses Flag-tagged full-length human Champ1 proteinDepositorInsertChamp1 (CHAMP1 Human)
UseTags2X Flag tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2
Plasmid#116965PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC
Plasmid#116964PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
RCASBP-Y CHA
Plasmid#11480DepositorInsertGateway ccdB reading frame cassette
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMarch 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-Rap2a-V5
Plasmid#149509PurposeStable expression of human Rap2a.DepositorInsertRAP2 (RAP2A Human)
UseLentiviralTagsV5ExpressionMutationCodon Optimized SequencePromoterUbcAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC
Plasmid#116966PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA17
Plasmid#48990Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW_RcCHAD-mCherry
Plasmid#127784PurposeRcCHAD-mCherry expression in plantsDepositorInsertUbi10p:RcCHAD-mCherry
UseTagsmCherryExpressionPlantMutationPromoterUbiquitin10 promoter + 5' UTRAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_RcCHAD
Plasmid#127782PurposeExpresses RcCHAD in E. coli cellsDepositorInsertRcCHAD
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-3
Plasmid#90327PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchA WT
Plasmid#87102Purposeexpresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosaDepositorInsertpchA WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-3xAla
Plasmid#233921PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xAla (mutated TRAF6 binding site)DepositorInsertCHARMS-3xAla
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-DHF
Plasmid#233922PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed Helical Filament)DepositorInsertCHARMS-DHF
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-1xA
Plasmid#233924PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutationDepositorInsertCHARMS-1xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRSV_CHARMS-3xA
Plasmid#233926PurposeMammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutationDepositorInsertCHARMS-3xA
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterSV40Available sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only