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Plasmid#188893PurposettTi5605 MosSci targeting vector with floxed Cbr-unc-119 markerDepositorTypeEmpty backboneUseMossci targeting vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pMLS715
Plasmid#188887PurposeMultisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos ArmDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS538
Plasmid#188881PurposeGateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)DepositorInsertCas9
UseGateway entry vectorTagsExpressionMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMLS415
Plasmid#114272PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS292
Plasmid#73725PurposeSapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmidDepositorInsert2xNLS-mCherry + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS7-egfp
Plasmid#112917PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPS7 promoter and Ps7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shREN
Plasmid#105583Purposeretrovirally express control shRNA with GFP markerDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLS-mir-17-92
Plasmid#64090Purposeexpresses mir-17-92DepositorInsertmiR-17-92 (MIR17HG Human)
UseTagsExpressionMammalianMutationPromoterMSCVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA h-MLSN
Plasmid#21083DepositorInsertTrpM1 (TRPM1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 N-MLSN
Plasmid#21082DepositorInsertTRPM1 (TRPM1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shTAF12.376
Plasmid#105578Purposeretrovirally express TAF12 shRNA with GFP markerDepositorInsertTAF12 shRNA #376
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shRPA3.457
Plasmid#105584Purposeretrovirally express RPA3 shRNA with GFP markerDepositorInsertRPA3 shRNA #457
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shTAF12.364
Plasmid#105577Purposeretrovirally express TAF12 shRNA with GFP markerDepositorInsertTAF12 shRNA #364
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MLS-HyPer7
Plasmid#136470PurposeMammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTandem mitochondrial targeting signal of cytochro…ExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
2MLS-HyperNova/pcDNA3
Plasmid#221457PurposeExpressing HyperNova in mitochondriaDepositorInsertHyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMLS-SV40-EGFP
Plasmid#46919PurposeTarget EGFP gene that is stably integrated into HEK293 genomeDepositorInsertGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_MLST8:1-326
Plasmid#211216PurposeMammalian expression of full-length human MLST8. N-terminal HiBiT tag.DepositorInsertMLST8:M1-G326 (MLST8 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MLS- mir-17-19b
Plasmid#64089Purposeexpresses a truncated version of mir-17-92 lacking miR-92DepositorInsertmir-17-19b (MIR17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
MLS-mir-17-92-Mut92
Plasmid#64094Purposeexpresses a mutated version of mir-17-92 (mutation of miR-92 seed)DepositorInsertmiR-17-92 (MIR17HG Human)
UseTagsExpressionMammalianMutationmutation of miR-92 seed sequencePromoterMSCVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm2_A
Plasmid#145881PurposeInsect Expression of DmLSm2DepositorInsertDmLSm2 (CG10418 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm3_A
Plasmid#145882PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm4_A
Plasmid#145883PurposeInsect Expression of DmLSm4DepositorInsertDmLSm4 (LSm-4 Fly)
UseTagsV5-6xHisExpressionInsectMutationtwo silent mutations compared to the sequence of …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm5_A
Plasmid#145884PurposeInsect Expression of DmLSm5DepositorInsertDmLSm5 (CG6610 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm7_A
Plasmid#145885PurposeInsect Expression of DmLSm7DepositorInsertDmLSm7 (LSm7 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
R777-E133 Hs.MLST8
Plasmid#70417PurposeGateway ORF clone of human MLST8 [NM_022372.4] with stop codon (for native or N-terminal fusions)DepositorInsertMLST8 (MLST8 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLS-mir-17-92-Mut20
Plasmid#64095Purposeexpresses a mutated version of mir-17-92 (mutation of miR-20 seed)DepositorInsertmiR-17-92 (MIR17HG Human)
UseTagsExpressionMammalianMutationmutation of miR-20 seed sequencePromoterMSCVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_MLST8:1-326
Plasmid#211290PurposeMammalian expression of full-length human MLST8. C-terminal HiBiT tag.DepositorInsertMLST8:M1-G326 (MLST8 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm6_G
Plasmid#146366PurposeInsect Expression of DmLSm6DepositorInsertDmLSm6 (CG9344 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm8_G
Plasmid#146367PurposeInsect Expression of DmLSm8DepositorInsertDmLSm8 (CG2021 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm1_A
Plasmid#145886PurposeInsect Expression of Dm-Lsm1DepositorInsertDm-Lsm1
UseTagsExpressionInsectMutationTwo silent mutation compared to the sequence give…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm2_A
Plasmid#145887PurposeInsect Expression of DmLSm2DepositorInsertDmLSm2 (CG10418 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm4_A
Plasmid#145889PurposeInsect Expression of Dm-Lsm4DepositorInsertDm-Lsm4
UseTagsExpressionInsectMutationone non silent mutation G127R compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm5_A
Plasmid#145890PurposeInsect Expression of DmLsm5DepositorInsertDmLsm5 (CG6610 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm7_A
Plasmid#145891PurposeInsect Expression of DmLSm7DepositorInsertDmLSm7 (LSm7 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm3_A
Plasmid#145888PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E134 Hs.MLST8-nostop
Plasmid#70418PurposeGateway ORF clone of human MLST8 [NM_022372.4] without stop codon (for C-terminal fusions)DepositorInsertNM_022372.4 (MLST8 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag
Plasmid#124915PurposeFor stable expression sgRNA resistant Flag tagging mLST8_DepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2pA2-mnx1:MLS-KillerRed
Plasmid#115515PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoter-3mnx1Available sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
COX8mls-Flag-APEX2-SOPP3
Plasmid#229075PurposeExpresses APEX2-SOPP3 in the mitochondrial matrixDepositorInsertExpress APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
YCe1885 HC_Kan_Kozak-ATG-MLS_p6
Plasmid#100676Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertKozak-ATG-MLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterUASAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-TAP mLST8 (aka: GbL)
Plasmid#12575DepositorInsertmLST8 (MLST8 Human)
UseRetroviralTagsTAPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut1
Plasmid#124916PurposeFor stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut2
Plasmid#124917PurposeFor stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only