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Showing: 21 - 40 of 715 results

  4. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  8. pKM263-USP7

    Plasmid
    #196365
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    USP7 catalytic domain
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only S207-E560
    Promoter
    T7
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196365-plasmid-map-sequence-id-386294

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  11. PRE-Hsp70BbCas9_1.2

    Plasmid
    #190796
    Purpose
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190796-plasmid-map-sequence-id-376571

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  17. MSP7-bio

    Plasmid
    #47735
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP7
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47735-plasmid-map-sequence-id-68747

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  19. pSP72 GSK3beta

    Plasmid
    #60125
    Purpose
    for transcription of GSK3beta
    Depositor
    Andrew McMahon
    Insert
    Glycogen synthase kinase 3 beta (Gsk3b Mouse)
    Use
    Cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60125-plasmid-map-sequence-id-307046

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Showing: 21 - 40 of 715 results