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Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
UseTagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastMutationPromoterTPI1Available sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJOG418
Plasmid#105342PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBKWH
Plasmid#133898PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMH120
Plasmid#40593PurposeExpresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expressionDepositorUseTagsExpressionYeastMutationPromoterADH1 and TEFAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS414 GPD-Sis1
Plasmid#18687DepositorInsertSis1 (SIS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSDMA66
Plasmid#128355PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorInsertNoureothricin (NAT)
UseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCP5
Plasmid#35397DepositorTypeEmpty backboneUseReporterTagsExpressionYeastMutationPromoterGDP promoterAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS22H
Plasmid#133904PurposeExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSDMA67
Plasmid#128356PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorInsertNoureothricin (NAT)
UseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F53
Plasmid#23077DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9RPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F30
Plasmid#23066DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 9 to Glutamine H3 K9QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F31
Plasmid#23067DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed lysine 14 to Glutamine K14QPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCK012
Plasmid#105344PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES262 [prADH1-Msn2-mCherry, pnh604]
Plasmid#103844PurposeYeast Expression PlasmidDepositorInsertpAdh1-Msn2-mCherry
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS314-MDM2
Plasmid#18654DepositorInsertMDM2 (MDM2 Human)
UseTagsGal1 promoterExpressionYeastMutationPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCP5b
Plasmid#37185DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-chNfasc (+RSLE)
Plasmid#89418PurposeTwo-hybrid systemDepositorInsertNFASC (NFASC Chicken)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc
Plasmid#133901PurposeExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F14
Plasmid#23063DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 deleted amino acids 3-29PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS414-54
Plasmid#23084DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysines 4 and 9 to Glutamines,…PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD100
Plasmid#23085DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 4 to Glutamine, H3 K4Q.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJD101
Plasmid#23086DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 4 to Arginine, H3 K4R.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS414-59
Plasmid#23087DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Serine 10 to Alanine, H3 S10A.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only