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Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_P_12_asn_mbfA
Plasmid#90289PurposeBB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2DepositorInsertmbfA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH520
Plasmid#102673PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH521
Plasmid#102674PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH517
Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTG07-Amp-FRT
Plasmid#128276PurposeGene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65DepositorInsertGal4-VVD-p65
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGKO2
Plasmid#63617Purposea binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.DepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO-N
Plasmid#128143PurposeDinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marinaDepositorInsertRifampin ADP-ribosylating transferase + potential promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOX-IWgfp
Plasmid#12244DepositorInserteGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoter of the Rat Insulin II gene is upstream o…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF-TAP.LIC.HXGPRT
Plasmid#83367PurposePlasmid contains LIC cassette upstream of an in-frame copy of SF-TAP and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertStrep-FLAG
UseToxoplasma gondiiTagsSF-TAPExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE46 dnWnt-1
Plasmid#16860DepositorInsertdnWnt-1 (Wnt1 Mouse)
UseXenopus expressionTagsExpressionMutationtruncated genePromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPV033
Plasmid#178359PurposeTargeted gene knock-in plasmid for Schizophyllum communeDepositorInsertsLeft flank
nour_cassette
Right flank
UseFungal homologous recombination plasmidTagsExpressionMutationPromoterS. commune gpd and n.a.Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPtac
Plasmid#166937PurposePtac single-level gene expression controller (SLC)DepositorInsertGenetic circuit expressing GFP- PTAC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC
Plasmid#166941PurposeDual control based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Dual Control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGKO1
Plasmid#63611Purposea binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.DepositorTypeEmpty backboneUseBinary fungal vectorTagsExpressionMutationPromoterLac PromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.ind
Plasmid#61284PurposeArabinose-inducible repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF101.3
Plasmid#134772PurposeA binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pYFP.LIC.DHFR
Plasmid#83114PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pknox2
Plasmid#74354PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pknox2. Not for gene expression.DepositorInsertPbx/knotted 1 homeobox 2 (Pknox2 )
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSLfa PUb TAL-KMO-L
Plasmid#52887PurposeLeft hand TALEN against Aedes aegypti KMO geneDepositorInsertTALEN-KMO
UseTagsExpressionMutationPromoterAe aegypti polyubiquitinAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBLIC-neo
Plasmid#45198Purposea ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBALU-ext
Plasmid#69339PurposeIt allows to extend the genomic insert in a fosmid.DepositorInsertkanamycin gene +pCC1FOS homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB1_P_12_asn_coxA
Plasmid#90290PurposeBB1 promoter coxA gene of Aspergillus niger between FS1 and FS2DepositorInsertcoxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBLIC-puro
Plasmid#45197Purposea ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFF19H
Plasmid#49783Purposeexpresses hygromycin resistance gene in plant cellsDepositorInsertHPH
UsePlant expressionTagsExpressionMutationPromoterCaMV 35SAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-Leum
Plasmid#44364DepositorInsertLeum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterLeum promoterAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
SJZ5-HPH
Plasmid#228258PurposeTemplate plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin BDepositorInserthph
UseTagsExpressionYeastMutationPromoterSSA1 S.c.Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
UseTagsExpressionYeastMutationPromoterTEF1 S.c.Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ2-TRP1
Plasmid#228256PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)DepositorInsertTRP1
UseTagsExpressionYeastMutationPromoterBBC1 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SJZ10-LEU2
Plasmid#228262PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)DepositorInsertLEU2
UseTagsExpressionYeastMutationPromoterLEU2 K.l.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSTAR2
Plasmid#166940PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Small transcription activating RNAs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAR
Plasmid#166939PurposeSTAR-based multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Small Transcription activating RNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS1sg
Plasmid#154341PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS1 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS2sg
Plasmid#154342PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS2 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CANTsg
Plasmid#154343PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCANT sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAPsg
Plasmid#154344PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAP sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTF101.4
Plasmid#134773PurposeA binary plasmid containing nopaline synthase promoter to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p249_LTJ_1kbFoxp3wtTemplate
Plasmid#82664Purpose1kb HDR template for murine wildtype Foxp3DepositorInsert1kb genomic DNA sequence from murine wildtype Foxp3
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only