We narrowed to 7,456 results for: Lif;
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Plasmid#139721PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, CPHDepositorInsertpMOD_A'_pSlH4:NnCPH:tSlH4
UseLuciferaseTagsExpressionPlantMutationPromoterSlH4Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NES-His-rsCaMPARI-mRuby3
Plasmid#120805PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus excludedDepositorInsertrsCaMPARI
UseAAVTagsNES-His and mRuby3ExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Plasmid#140153PurposeExpresses a regulatable vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-Nme2_tracr
Plasmid#192146PurposeExpresses Nme2-sgRNA cloned with BspQIDepositorInsertNme2 sgRNA scaffold
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATM N-terminal sgRNA
Plasmid#207089PurposepX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.DepositorInsertATCATTAAGTACTAGACTCA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin flexed SV-tag
Plasmid#163686Purposeexpress SV-Tag construct in a Cre-dependent mannerDepositorInsertsynaptophysin 9X HA
UseAAVTags9X HAExpressionMammalianMutationPromotersynapsinAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
P527
Plasmid#139741PurposeIndividual component of Caffeic Acid biosynthesis pathway, RcTALDepositorInsertTAL
UseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL12-GCaMP6f-pHuji bb118
Plasmid#158981PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.DepositorInsertGCaMP6f-pHuji
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833 a…PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP34
Plasmid#65512PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pQIq_MRGS_HIS8_(27G2)_FLAG
Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCO604
Plasmid#139737Purposeindividual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3HDepositorInsertH3H
UseLuciferase; Moclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP37
Plasmid#65515PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-Cer-cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL13-MgtC bb118
Plasmid#158982PurposeConstitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.DepositorInsertmgtC
UseTagsExpressionBacterialMutationPromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP36
Plasmid#65514PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-miniTurboID-EGFP
Plasmid#163847PurposeIn vitro transcription of miniTurboID-EGFP. Parton lab clone JRTDepositorInsertminiTurbo
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-muSOX
Plasmid#131701Purposeexpresses muSOX with GFP N-terminally fusedDepositorInsertGFP-muSOX (GAMMAHV.ORF37 MHV68)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin SV-tag
Plasmid#163685Purposeexpress SV-Tag construct in a Cre-independent mannerDepositorInsertsynatophysin 9X HA tag T2A tdtomato
UseAAVTags9X HAExpressionMammalianMutationPromotersynapsinAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorInsertTLN1 (TLN1 Human)
UseTagsHis6 (tev cleavage)ExpressionBacterialMutationPromoterLacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCO486
Plasmid#139729PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A_35S Short_TMV 5':AsNpga:tAtug7
UseLuciferaseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRB
Plasmid#104475PurposePlasmid carrying FRB with C-terminal HA tag.DepositorInsertFRB (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-deltaR8.2(A14C,E45C,A92E)
Plasmid#79048Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN1-fDIO-rtTA
Plasmid#166598PurposeEncodes Flp-dependent rtTADepositorInsertrtTA
UseAAVTagsExpressionMutationPromoterhuman synapsin 1Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gag-delta-p6-mEGFP
Plasmid#116890Purposeexpresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cellsDepositorInsertsynthetic Gag missing the p6 domain
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
P366
Plasmid#139722PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A''_pSlRBCS2:AsNpga:tAtuG7
UseLuciferaseTagsExpressionPlantMutationPromoterSlRBCS2Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
hygII-UT-ABAleon2.15
Plasmid#106983PurposeABAleon2.15 in plant expression vector (Hygromycin resistance)DepositorInsertABAleon2.1
UseTagsExpressionPlantMutationPromoterUBQ10Available sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVermilion-C1
Plasmid#105756PurposeEmpty vector for creating N terminal mVermilion fusionsDepositorTypeEmpty backboneUseTagsmVermilionExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCO487
Plasmid#139730PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, H3HDepositorInsertpMOD_B_AtUbi10:NnH3H:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx2
Plasmid#104473PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx2-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCO488
Plasmid#139731PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, HispsDepositorInsertpMOD_C'_CmYLCV:NnHisps:tHsp
UseLuciferaseTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_( E3_5)_FLAG
Plasmid#199612PurposeEncodes DARPin-FLAG E3_5 for bacterial expression and column purificationDepositorInsertDARPin-FLAG E3_5 (control)
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pF(UG) CamKII sf iGluSnFR
Plasmid#158769PurposeiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.DepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationAdditional ER and Golgi export sequencesPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM-ENO2-mCherry-YFP
Plasmid#180570PurposeDual fluorescent reporter for 5' UTR activity in yeast mCherry/YFPDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGM-ENO2-YFP-mCherry
Plasmid#180569PurposeDual fluorescent reporter for 5' UTR activity in yeast YFP/mCherryDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-NLS-TurboID
Plasmid#163849PurposeIn vitro transcription of nls-TurboID. Parton lab clone JSTDepositorInsertTurboID
UseIn vitro transcription of rnaTagsnlsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRBx2
Plasmid#104476PurposePlasmid carrying FRBx2 with C-terminal HA tag.DepositorInsertFRBx2 (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
P442
Plasmid#139728PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsiveDepositorInsertpMOD_D_pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterAtRAB18Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only