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We narrowed to 24,010 results for: Nes

Showing: 951 - 1000 of 24010 results

  8. T40A+Q54A

    Plasmid
    #217913
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP T40A+Q54A
    Use
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Threonine at position 41 and 253 mutated to Alani…
    Promoter
    T5
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217913-plasmid-map-sequence-id-437015

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  10. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid
    #123354
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    Use
    Tags
    C-terminal ER retention signal (KDEL), cp-moxCeru…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    123354-plasmid-map-sequence-id-311156

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  11. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

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  13. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

    Plasmid
    #123344
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-CKAR(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123344-plasmid-map-sequence-id-307096

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  14. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid
    #123336
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Cerulean3-cpCerulean3-FLARE-AKAR
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123336-plasmid-map-sequence-id-307044

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  24. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  25. pAAV-hSyn-GRAB_NE1h

    Plasmid
    #123309
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1h
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123309-plasmid-map-sequence-id-240668

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  26. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  27. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

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  30. pETM33_Nsp15

    Plasmid
    #156481
    Purpose
    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156481-plasmid-map-sequence-id-307733

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  31. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

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  32. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  34. pETM33_Nsp4

    Plasmid
    #156474
    Purpose
    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156474-plasmid-map-sequence-id-307734

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  36. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  37. pLenti PGK mKate2-OGT K842M (Puro)

    Plasmid
    #154291
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    K842M mutation (Catalytic Inactive)
    Promoter
    PGK
    Available since
    Dec. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154291-plasmid-map-sequence-id-332432

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  38. pAAV-hSyn-GRAB_NEmut

    Plasmid
    #123310
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE control sensor GRAB_NEmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123310-plasmid-map-sequence-id-240667

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  43. lentiCas9-Venus

    Plasmid
    #70267
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    Cas9
    Venus Reporter
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS and P2A
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70267-plasmid-map-sequence-id-126420

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  44. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

    Enlarge

  45. lentiCRISPR - EGFP sgRNA 4

    Plasmid
    #51763
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51763-plasmid-map-sequence-id-79382

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  47. lentiCRISPR - EGFP sgRNA 5

    Plasmid
    #51764
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51764-plasmid-map-sequence-id-79386

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  50. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Article
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

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Showing: 951 - 1000 of 24010 results