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pDONR221-SLC7A7_STOP

Plasmid

#161387

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A7 (SLC7A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PGKp-GFP-YAP (5SA)

Plasmid

#174174

Purpose

Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Five LATS phosphorylation sites (S61, S109, S127,…

Promoter

PGK

Available since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EFSp-GFP-YAP (5SA)

Plasmid

#174170

Purpose

Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Five LATS phosphorylation sites (S61, S109, S127,…

Promoter

EFS

Available since

Sept. 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GST-CLCb

Plasmid

#47404

Depositor

Peter McPherson

Article

Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

Insert

CLCb

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg2

Plasmid

#218531

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PKG I alpha WT

Plasmid

#16390

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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PKG I beta WT

Plasmid

#16394

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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PKG I alpha CAT

Plasmid

#16392

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, S65D

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
PKG I alpha dNPS

Plasmid

#16391

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: delet…

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-AKAR4-NES

Plasmid

#64727

Purpose

Cytosol-targeted, FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

Insert

AKAR4-NES

Use

Tags

Cerulean, NES, and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pECE-HA-FOXO3 S574A

Plasmid

#52426

Purpose

mammalian expression of FOX03 with mutant JNK phosphorylation site

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S574A, mutant JNK phosphorylation site

Promoter

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pECE-HA-FOXO3 R248K R250K

Plasmid

#52427

Purpose

mammalian expression of FOXO3 methylation resistant mutant

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R248K R250K, mutant methylation sites

Promoter

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
ShadowY-CLC

Plasmid

#193560

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CLC-ShadowY

Plasmid

#193561

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge