We narrowed to 1,752 results for: pyogenes Cas9

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-GFP

Plasmid

#86145

Purpose

Lentiviral vector expressing Cas9-GFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

Cas9-GFP

Use

CRISPR and Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Article

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 VPR

Plasmid

#188511

Purpose

Expresses FLAG tagged SpRY dCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 KRAB

Plasmid

#188501

Purpose

Expresses FLAG tagged SpRY dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAH-CTX1-rhadCas9

Plasmid

#129391

Purpose

Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Burkholderia cenocepacia codon-optimized dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Entire dCas9 codon optimized for the GC-rich B. c…

Promoter

PrhaBAD

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAT15547_PEmax-SuperFi-Cas9

Plasmid

#184377

Purpose

Mammalian expression of SuperFi-Cas9 PEmax prime editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

PEmax-SuperFi-Cas9

Use

CRISPR

Tags

SV40 NLS and c-myc NLS

Expression

Mammalian

Mutation

H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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BII-gR-BtW-eSpCas9

Plasmid

#133358

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dSpCas9

Plasmid

#92113

Purpose

Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Cbh

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAT15546_nABE8e-SuperFi-Cas9

Plasmid

#184376

Purpose

Mammalian expression of SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAT15542_nCBE-SuperFi-Cas9

Plasmid

#184372

Purpose

Mammalian expression of SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15544_nABE-SuperFi-Cas9

Plasmid

#184374

Purpose

Mammalian expression of SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 Synthetic, S. pyogenes)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAT15541_dCBE-SuperFi-Cas9

Plasmid

#184371

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15543_dABE-SuperFi-Cas9

Plasmid

#184373

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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