We narrowed to 5,107 results for: PEP

1. pCNL-C1_PTS1

   Plasmid

   #65700

   Purpose

   Expresses CNL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pONL-C1_PTS1

   Plasmid

   #65701

   Purpose

   Expresses ONL-PTS1 in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   peroxisomal targeting signal 1 (tripeptide SKL)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-20b-T7-Ntx

   Plasmid

   #58714

   Purpose

   Expresses T7-Notexin in Escherichia coli

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Ce25

   Plasmid

   #51296

   Purpose

   Expresses a plant arginyl-tRNA synthetase in E.coli

   Depositor

   Gabor Igloi

   Article

   Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7

   Insert

   arginyl-tRNA synthetase
   

   Use

   Tags

   His Tag and Thioredoxin

   Expression

   Bacterial

   Mutation

   GTT (Val) insertion after initiation codon to per…

   Promoter

   T7

   Available since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PcDNA-DEST53-CypB-Del1 N-term GFP

   Plasmid

   #36131

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 37-216 of cyclophilin B; F21…

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pEG593

   Plasmid

   #40119

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
   

   Use

   Gateway destination vector

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   R274E, K318E, Exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pEG628

   Plasmid

   #40120

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
   

   Use

   Gateway destination vector

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   contains aa1-244, Exon 2 recoded to be RNAi-resis…

   Promoter

   4.4 kb mex-5 promoter fragment

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pBS ADAMTS3

   Plasmid

   #19205

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   ADAM metallopeptidase with thrombospondin type 1 (ADAMTS3 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pAcUW51-CgE

   Plasmid

   #13762

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

   Insert

   HSV-1 glycoprotein E
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   C-terminal region of the HSV-1 gE (KOS strain ect…

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid

    #158768

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Pgk1

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

    Plasmid

    #126705

    Purpose

    donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

    Plasmid

    #111585

    Purpose

    An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myriRFP670V5-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS117 His14-Avi-45xGS-anti GFP nanobody

    Plasmid

    #199370

    Purpose

    Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-HLA-A2

    Plasmid

    #135504

    Purpose

    Mammalian expression of myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV9retro

    Plasmid

    #224687

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    Zhigang He

    Article

    Zhang et al Cell. 2024 May 3:S0092-8674(24)00447-1.

    Insert

    Rep2/Cap9retro
    

    Use

    AAV

    Tags

    Expression

    Mutation

    insertion of 10-mer peptide from AAV2retro (LADQD…

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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