170,823 results

Showing: 4001 - 4020 of 170823 results

Results per page:

Sort results by:

pBR_attB(bxb)_lox

Plasmid

#183762

Purpose

Bxb1-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-CMV-AfmW3A

Plasmid

#133402

Purpose

Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Afamin and Wnt3a (Afm, Wnt3a Mouse)

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLminP_Luc2P_RE4

Plasmid

#90338

Purpose

NRF2 - Antioxidant response element gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NRF2

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-hTLR4-VenusFlag

Plasmid

#197640

Purpose

Human TOLL protein expression in mammalian cells

Depositor

Haian Fu

Insert

TLR4 (TLR4 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmVen-TopBP1

Plasmid

#211413

Purpose

mammalian expression of human TopBP1 N-terminally fused to mVenus

Depositor

Helmut Pospiech

Insert

TopBP1 (TOPBP1 Human)

Tags

mVenus

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Neo

Plasmid

#127762

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab7A

Plasmid

#61803

Purpose

Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab7A (RAB7A Human)

Tags

2x HA and GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TO-hNIL

Plasmid

#172113

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST_FLAG-HUWE1

Plasmid

#187120

Purpose

mammalian cell expression of FLAG-HUWE1

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1+_hsTRPA1_myc_dTom

Plasmid

#183179

Purpose

myc tagged hsTRPA1 used for cell transfection

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Inserts

hsTRPA1 (TRPA1 Human)
dTom

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge