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pJT11

Plasmid

#98308

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

TRPL3ClLIS1>TRPL41B

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPathBrick

Plasmid

#65006

Purpose

E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive native promoters

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Tags

FLAG and GST

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCB59 pDEST crybb1:mKate2

Plasmid

#195986

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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6xAP-1_RE::Renilla

Plasmid

#178317

Purpose

AP-1 Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

AP-1 Renilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE 3xHA-MKK6-EE puro

Plasmid

#216791

Purpose

Mammalian expression of constitutively active human MKK6

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

MKK6 (MAP2K6 Human)

Use

Retroviral

Tags

3x-HA

Expression

Mammalian

Mutation

S207E and T211E, constitutively active

Promoter

LTR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H2

Plasmid

#79513

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR BETA (NR1H2 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pP19 (GB0038)

Plasmid

#68195

Purpose

Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMC-6-BeYDV-Rep-RepA-9

Plasmid

#197715

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV Rep/RepA proteins: native ORFs

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rap1

Plasmid

#134928

Purpose

Fluorescently tagged GTPase.

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

Rap1B, Cerulean

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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Rap1A FLARE

Plasmid

#134926

Purpose

Rap1A biosensor

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

Rap1A, mCerulean3, RaIGDS RBD, YPet

Expression

Mammalian

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-nlsABACUS2-400n

Plasmid

#203726

Purpose

Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

Depositor

Alexander Jones

Article

Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

Insert

nlsABACUS2-400n

Use

Synthetic Biology

Tags

Myc

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Renilla:Tnos (GB0109)

Plasmid

#68215

Purpose

Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSNAP-CLC-SNAP

Plasmid

#38012

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

SNAP26m

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCB24 pDEST crybb1:TagBFP

Plasmid

#195987

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-TWIST2 neo

Plasmid

#197936

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::FLuc

Plasmid

#178316

Purpose

P53 Firefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

P53 FLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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