We narrowed to 13,031 results for: Hal
-
Plasmid#128945Purposeexpresses Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
TagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Clover-TOMM20-N-10
Plasmid#56307PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515DepositorAvailable SinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
Plasmid#182359PurposePositive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.DepositorInsertUltra-pan-neuronal positive control pUNP::gfp(NLS)
ExpressionWormPromoterPromoter Ultra Pan NeuronalAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:: HA-hM4Dnrxn
Plasmid#52525PurposeExpresses N-terminal HA tagged hM4DnrxnDepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM33_WDR5_WD
Plasmid#178491PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-tdTomato-f
Plasmid#127092PurposeAn AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomatoDepositorInserttdTomato-f
UseAAVTagsfarnesylation signal from c-Ha-RasExpressionMammalianAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CRY2
Plasmid#140572PurposeFor use in light-induced protein inactivation through the interaction with CIB1DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2
Plasmid#10821DepositorInsertArgonaute 2 (AGO2 Human)
TagsFLAG and HAExpressionMammalianMutationR668H. Additionally, N terminus different from wi…Available SinceOct. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1-M7A
Plasmid#159164PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1-M7A
TagsCOX4 pre-sequenceExpressionYeastMutationMutated Met 7 of the cyt b562 module of mito-yHS1…PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pORANGE Cplx2-GFP KI
Plasmid#131476PurposeEndogenous tagging of Complexin2: C-terminal (amino acid position: L128)DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTphiC31
Plasmid#127535PurposePlasmid encodes A. thaliana codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCas9-sgCh2-2-Blast
Plasmid#199645PurposeExpresses Cas9 and sgRNA control guide targeting intergenic regionDepositorInsertN/A
UseLentiviralAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-TOMM20-N-10
Plasmid#55913PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600DepositorAvailable SinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Flag-YAP-S163/164A
Plasmid#33089DepositorAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-1Cre
Plasmid#98038PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
ExpressionMammalianPromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT13
Plasmid#127534PurposePlasmid encodes A. thaliana codon optimized Integrase 13.DepositorInsertIntegrase 13 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
ExpressionWormPromoterodr-3Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4EGFP
Plasmid#98037PurposeSecond-generation rabies viral vector genomeDepositorInsertEGFP
ExpressionMammalianPromoterCMVAvailable SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE
Plasmid#140228PurposeRRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.DepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseLentiviralTagsBFPExpressionMammalianPromoterRRM2 endogenous promoterAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PHR-iSH-T2A-CIBNcaax
Plasmid#173860PurposeMammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)DepositorInsertmCherry-PHR-iSH-T2A-CIBNcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCAGAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATG --> TGTTGGAGAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP-shACTN2
Plasmid#52677PurposeshRNA against α-actinin-2 with eGFP transfection markerDepositorAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-p65
Plasmid#183929PurposeOptogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposureDepositorAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE062
Plasmid#176819PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert701
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW683 - pAHP6>>
Plasmid#115998Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyn-iTrkA-mCh-CRY2
Plasmid#106169Purposemammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)DepositorAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATA --> TGTTGGAAAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
ExpressionMammalianPromoterTRE-tight and hEF1aAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)
Plasmid#114102Purposefusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGLucM23-iChloC-EYFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KPNA4_Arm
Plasmid#178495PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSRW1JZ
Plasmid#129528PurposeC. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pairDepositorInsertFlincG3
ExpressionWormAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-a2-GEMS(JAK-STAT)
Plasmid#209110PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)DepositorInsertA-a2-GEMS(JAK-STAT)
ExpressionMammalianAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G'-IRES-EmGFP
Plasmid#209127PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFPDepositorInsertSUMO-A'-l4-G'-IRES-EmGFP
UseLentiviralExpressionMammalianAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l4-G-IRES-EmGFP
Plasmid#209126PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFPDepositorInsertSUMO-A'-l4-G-IRES-EmGFP
UseLentiviralExpressionMammalianAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHLdeltaB-IRES-GFP
Plasmid#187642PurposeMutant VHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralExpressionMammalianMutationdeletion of amino acids 91 to 121Available SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgSHOC2-1
Plasmid#86128PurposeLentiviral vector expressing Cas9 and an sgRNA targeting SHOC2DepositorAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only