4,142 results

1. pRK5-myc-Rac1-T17N

   Plasmid

   #12984

   Depositor

   Gary Bokoch

   Insert

   Rac1 DN (RAC1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR/pSUPER+ (575-1)

   Plasmid

   #17338

   Purpose

   Gateway entry vector for shRNA expression under H1 promoter

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   RNAi; Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. piRFP702-N1

   Plasmid

   #45456

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP702
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5 mouse src K295R Y527F

   Plasmid

   #13657

   Depositor

   Peter Howley, Joan Brugge

   Insert

   src K295R Y527F (Src Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K295R Y527F. Dominant negative mutant.

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CaMPARI V398D

   Plasmid

   #60786

   Purpose

   Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Eric Schreiter, Loren Looger

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI V398D
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   V398D

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-RhoA FLARE.sc Biosensor T19N

   Plasmid

   #12152

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor T19N (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   RhoA T19N (dominant-negative mutation)*

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-neo largeTgenomic

    Plasmid

    #10891

    Depositor

    William Hahn

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA-HA-Nedd4/2795

    Plasmid

    #11426

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Nedd4-1 (Nedd4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D661N compared to GenBank reference sequence U966…

    Promoter

    CMV

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSV Sport PPAR gamma 2

    Plasmid

    #8862

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FCK-Arch-GFP

    Plasmid

    #22217

    Purpose

    Lentiviral expression of Arch-GFP for optogenetic inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Arch
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGKneolox2DTA.2

    Plasmid

    #13449

    Depositor

    Philippe Soriano

    Insert

    PGKneolox2DTA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSV Sport PPAR gamma 1

    Plasmid

    #8886

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. siSlug3

    Plasmid

    #10905

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    siSlug (SNAI2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge