4,180 results

1. MSP2443

   Plasmid

   #72252

   Purpose

   Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-CRY2-CreN

   Plasmid

   #26888

   Purpose

   Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2-CreN (cre, CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   aa 19-104 of Cre

   Promoter

   CMV

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLM292

   Plasmid

   #21873

   Depositor

   Keith Joung

   Article

   Foley et al Nat Protoc. 2009. 4(12):1855-67.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLM290

   Plasmid

   #21872

   Depositor

   Keith Joung

   Article

   Foley et al Nat Protoc. 2009. 4(12):1855-67.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag Snail 6SA

   Plasmid

   #16221

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S96A, S100A, S107A, S111A, S115A, S119A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pARE-lux

   Plasmid

   #11768

   Depositor

   Jeff Wrana, Joan Massague

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   ARE
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Stat3 S727A pRc/CMV

   Plasmid

   #8708

   Depositor

   Jim Darnell

   Article

   Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

   Insert

   Stat3 S727A (Stat3 Mouse)
   

   Expression

   Mammalian

   Mutation

   S727A (exhibits o serine phosphorylation either c…

   Available Since

   Aug. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGMKOSiE

   Plasmid

   #20865

   Depositor

   Keisuke Kaji

   Article

   Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

   Insert

   myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
   

   Expression

   Mammalian

   Available Since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1(-) mouse C/EBP delta

   Plasmid

   #12559

   Depositor

   Peter Johnson

   Article

   McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

   Insert

   CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
   

   Expression

   Mammalian

   Mutation

   S2G introduced during cloning

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Available Since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. miR-146a

    Plasmid

    #15092

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    pre-miR-146a
    

    Expression

    Mammalian

    Available Since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAd-Track Flag-SIRT1

    Plasmid

    #8438

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. FCK-Halo-GFP

    Plasmid

    #14750

    Purpose

    Lentiviral expression of Halo-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al PLoS One. 2007 Mar 21;2(3):e299.

    Insert

    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N. pharaonis halorhodopsin is mammalian codon-opt…

    Promoter

    CaMKIIa

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHB9-EGFP (Hb9 promoter) [TJ#96]

    Plasmid

    #16275

    Depositor

    Thomas Jessell

    Article

    Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

    Insert

    HB9 promoter
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1 puro PTEN shRNA

    Plasmid

    #10669

    Depositor

    William Hahn

    Insert

    PTEN shRNA (PTEN Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-myc-Rac1-wt

    Plasmid

    #12985

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-Sall4

    Plasmid

    #13361

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    sal-like 4 (Drosophila) (Sall4 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    *(see below)

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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