19,635 results

1. mVenus N1

   Plasmid

   #27793

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus N1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Raptor_1 shRNA

   Plasmid

   #1857

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HDAC2-H141A

   Plasmid

   #115346

   Purpose

   Expresses mouse HDAC2 cDNA with H141A point mutation

   Depositor

   Javier Gonzalez-Maeso

   Article

   Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

   Insert

   Histone deacetylase 2 (Hdac2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H141A

   Promoter

   CMV

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-) MID49 4xMyc Hisx6

   Plasmid

   #44596

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   MiD49 (Mief2 Mouse)
   

   Tags

   4xMyc and 6xHis

   Expression

   Mammalian

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Jim Wells, Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Human, Synthetic)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-BETAipi

   Plasmid

   #53277

   Purpose

   Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoter

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

   Plasmid

   #225708

   Purpose

   Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV9

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Promoter

   EF1α

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. P307-FBP_6

   Plasmid

   #139697

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM1190

    Plasmid

    #69994

    Purpose

    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Expression

    Bacterial

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid

    #219565

    Purpose

    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    

    Use

    Unspecified

    Promoter

    EF-1a

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-D1ER

    Plasmid

    #36325

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1ER second generation cameleon (calcium sensor) targeted to ER
    

    Tags

    KDEL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-KG-GST-YAP

    Plasmid

    #33052

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABEpuro-ERBB2 A775_G776insYVMA

    Plasmid

    #40982

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A775_G776insYVMA

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. 12XCSL-d1EGFP

    Plasmid

    #47684

    Purpose

    Notch reporter

    Depositor

    Urban Lendahl

    Article

    Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

    Insert

    TP-1 promoter
    

    Use

    Notch reporter construct

    Tags

    d1EGFP

    Mutation

    hexamerized 50-bp EBNA2 response element of the T…

    Promoter

    NA

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mscv2.2-NAIP5

    Plasmid

    #60205

    Purpose

    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

    Depositor

    Russell Vance

    Article

    Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

    Insert

    NAIP5
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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