162,619 results

1. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PP7-2xmCherry-CAAX

   Plasmid

   #74925

   Purpose

   Expresses PP7-2xmCherry-CAAX

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-2xmCherry-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.2 V5-DEST 3XFlag-SRSF3

   Plasmid

   #46736

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   SRSF3 (SRSF3 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

   Plasmid

   #192453

   Purpose

   intronized ttLbCas12a for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Tags

   Expression

   Mutation

   temperature-tolerant mutation (D156R)

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

   Plasmid

   #182251

   Purpose

   Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

   Plasmid

   #184464

   Purpose

   Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDual-IN-SMN1

   Plasmid

   #215721

   Purpose

   dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN1 EXON 7 (SMN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRMC2

   Plasmid

   #68940

   Purpose

   tetracycline-inducible expression vector for Staphylococcus aureus

   Depositor

   Tim Foster

   Article

   Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

   Type

   Empty backbone

   Use

   Tet inducible e. coli/staphylococcus shuttle vect…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Halo-DNAPKcs

    Plasmid

    #210787

    Purpose

    Plasmid for expression of HaloTag-DNA-PKcs in mammalian cells

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    DNA-PKcs
    

    Use

    Tags

    3xFLAG-HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_MAPK1_WT

    Plasmid

    #82145

    Purpose

    Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. plex_306-ccdB-hygro

    Plasmid

    #201135

    Purpose

    pLEX_306 plasmid with hygro marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB_TRE3G_scFV-sfGFP-Dot1LCD

    Plasmid

    #235582

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dot1l (Dot1l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. T7_CC_PEmax_IVT_Template

    Plasmid

    #216793

    Purpose

    Template for in vitro transcription of PEmax.

    Depositor

    Daniel Bauer

    Article

    Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

    Insert

    PEmax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2R

    Plasmid

    #167932

    Purpose

    lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    3' UTR only

    Promoter

    SP6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCGN-MEF2B

    Plasmid

    #32959

    Purpose

    For mammalian expression of human MEF2B with an HA tag

    Depositor

    Ron Prywes

    Article

    Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

    Insert

    MEF2B (MEF2B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCap-101-Apr

    Plasmid

    #227507

    Purpose

    Capture plasmid with apramycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV SOCS-3

    Plasmid

    #11486

    Depositor

    Ronald Kahn

    Article

    Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

    Insert

    SOCS-3 (Socs3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUltra-Plus

    Plasmid

    #161775

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Type

    Empty backbone

    Use

    Lentiviral; Multicistronic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-Lrrc59

    Plasmid

    #226565

    Purpose

    Encodes Lrrc59 protein laebeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge