164,577 results

1. pAAV-Puro_siKO-2TO

   Plasmid

   #86697

   Purpose

   Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 2TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHM830

   Plasmid

   #20702

   Depositor

   Thomas Stamminger

   Article

   Sorg et al Biotechniques. 1999 May . 26(5):858-62.

   Insert

   GFP-MCS-LacZ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK444 (EF1-OsTIR1(F74G) Puro)

   Plasmid

   #214374

   Purpose

   EF1-OsTIR1(F74G) (PiggyBac)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   EF1-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCMV-VSVgSS-TM

   Plasmid

   #207320

   Purpose

   Allows cloning of protein coding sequences to be incorportated into envelope of virions

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-EGFP-CAAX

   Plasmid

   #208683

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT-WT-HA-GFP11

   Plasmid

   #177719

   Purpose

   ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6

   Plasmid

   #170195

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   Nov. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST26-MSRA-C-HA

    Plasmid

    #195177

    Purpose

    Vector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    MSRA (MSRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111924

    Purpose

    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BFP-Rab5

    Plasmid

    #49147

    Purpose

    mammalian expression of BFP-Rab5 fusion

    Depositor

    Gia Voeltz

    Article

    Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

    Insert

    Rab5 (RAB5B Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_PIK3CB_WT

    Plasmid

    #82221

    Purpose

    Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-167

    Plasmid

    #118579

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-TRIM24

    Plasmid

    #28138

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-Lipo

    Plasmid

    #129237

    Purpose

    Protein expression in E.coli

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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