We narrowed to 31,489 results for: REP

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pCX-SpiCee-Kras

Plasmid

#140838

Purpose

mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee-Kras

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

IRFP and LEXY

Expression

Insect

Promoter

maternal tubulin

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.1-c002

Plasmid

#173087

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N369, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_VNp-mNeongreen_CydAB-mCherry

Plasmid

#182391

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
cydAB-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

Plasmid

#62955

Purpose

Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD

Plasmid

#65010

Purpose

NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

Depositor

Wensheng Wei

Article

Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

Inserts

rtTA-MAVS(C)
2A-linked delta-TK

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Tight-TRE

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
2xCBX7CD_W35A-IFP2.0C

Plasmid

#215755

Purpose

Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD_W35A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.2-c002

Plasmid

#173088

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N372, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase

Plasmid

#163404

Purpose

Expresses BMPR-2 lacking the kinase domain in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-delta59-68

Plasmid

#104361

Purpose

Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

deleted amino acids 59 through 68

Promoter

CMV

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ-mNeongreen

Plasmid

#182392

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-LZ-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

Plasmid

#159283

Purpose

A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Human INS sgRNA
Human INS guide RNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET32a-hEPR

Plasmid

#199233

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proepiregulin preproprotein (EREG Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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