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pET-45b-NFYC-IDR-mEGFP

Plasmid

#185015

Purpose

Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

NFYC IDR (Nfyc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL

Plasmid

#23103

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pGSTag Bim EL (Bcl2l11 Mouse)

Use

Tags

GSTag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
His-ZZ-TEV-BICDN(1-400)-GFP

Plasmid

#111862

Purpose

C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDN(1-400)-GFP (Bicd2 Mouse)

Use

Tags

8xHis tag, GFP, and ZZ tag

Expression

Insect

Mutation

Codon optimized for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLCHKO_Ptbp_ex8_3

Plasmid

#155083

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(1.3)-L4

Plasmid

#32577

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 1.3kb version

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

Plasmid

#102422

Purpose

Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

Tet1 catalytic domain mutant (Tet1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

H1620Y and D1622A mutations in Tet1 catalytic dom…

Promoter

TetO-CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Ring1A

Plasmid

#1994

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Use

Tags

Expression

Insect

Mutation

Kozak before start codon

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL(TD)

Plasmid

#23092

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Aspartate

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
Sema3f(L,+)-Fc-His

Plasmid

#72147

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-3 [N278/19R]

Plasmid

#114476

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-3 (Mouse). Derived from hybridoma N278/19.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-3 (Mus musculus) recombinant mouse monoclonal antibody (Syt3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBacPAK p110b WT

Plasmid

#55724

Purpose

His3 GST tagged WT p110 beta

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Use

Tags

His3 GST

Expression

Mutation

Promoter

polyhedrin promoter

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Expression

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Purpose

Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3 HC

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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CLIC6_pCSdest

Plasmid

#53789

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CLIC6

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MigR1-Flag-TRIB2

Plasmid

#131154

Purpose

Retroviral expression of flag-tagged mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ1371_sgNanog

Plasmid

#63710

Purpose

Express sgNanog in mammalian cells of mice.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgNanog, CMV promoter, puro, mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-MED31

Plasmid

#15427

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLV V5-nurim

Plasmid

#175110

Purpose

Lentiviral expression of V5-tagged mouse Nrm

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Nrm (Nrm Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Sema6b(L)-Fc-His

Plasmid

#72165

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaN)

Plasmid

#110189

Purpose

Expression of mouse MeCP2 (deltaN) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta N terminus

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SynDIG1/Tmem90b [L42/17R]

Plasmid

#128632

Purpose

Mammalian Expression Plasmid of anti-SynDIG1/Tmem90b (Mouse). Derived from hybridoma L42/17.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SynDIG1/Tmem90b (Mouse) recombinant mouse monoclonal antibody (Syndig1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-Fc-His

Plasmid

#72141

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-puro-5A, 6A mMCL1-OM

Plasmid

#45818

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

5A, 6A mMCL-1-OM

Use

Tags

Expression

Mammalian

Mutation

Changed Arginine to Alanine at 5th and 6th amino …

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
CAG-RORb2-IRES-GFPd2

Plasmid

#73998

Purpose

Plasmid expressing RORb2-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K24,83,88,104A

Plasmid

#58408

Purpose

Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
Anti-Homer1 [L113/28R]

Plasmid

#188189

Purpose

Mammalian Expression Plasmid of anti-Homer1 (Mouse). Derived from hybridoma L113/28

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Homer1 (Mus musculus) recombinant mouse monoclonal antibody (Homer1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TrpV3 [N15/39R]

Plasmid

#188210

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/39

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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