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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pC178: pAAV.U6.SapI.CMV.Cas13bt3

Plasmid

#204215

Purpose

Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

Cas13bt3 and U6.SapI sgRNA cloning site

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Codon optimisation by GenScript tool

Promoter

CMV/U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-ARHGEF2

Plasmid

#207959

Purpose

Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EBF1

Plasmid

#96965

Purpose

Dox-inducible expression of Ebf1 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

Ebf1 (Ebf1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pShuttle-Flag-XBP1u

Plasmid

#63679

Purpose

Adenoviral shuttle vector for XBP1u expression

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

unspliced

Promoter

CMV

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
IL6R PLAK1

Plasmid

#161518

Purpose

Expresses IL6 receptor (full length) in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

Interleukin 6 Receptor (IL6R Human)

Use

Lentiviral

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTreTight-Htt94Q-CFP

Plasmid

#23966

Depositor

Nico Dantuma

Article

Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

Insert

N-terminal fragment Huntingtin Q94 (HTT Human)

Tags

CFP

Expression

Mammalian

Mutation

94 polyQ repeat

Available Since

April 15, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Myc-SCAP

Plasmid

#134285

Purpose

Expresses Myc-tagged SCAP in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SCAP (Scap, Scap Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Article

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

Plasmid

#107581

Purpose

B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pdh promoter Bacillus megaterium DSM319

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/tom20-pbira(79-c)-ha

Plasmid

#154456

Purpose

expresses tom20-pbira(79-c)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

tom20-pbira(79-c)-ha (TOMM20 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pbira(1-g78)-sec61b

Plasmid

#154455

Purpose

expresses flag-pbira(1-g78)-sec61b/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pbira(1-g78)-sec61b (SEC61B Human)

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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