59,540 results

1. pFlagCMV2-UbcH8

   Plasmid

   #12442

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   UbcH8 (UBE2L6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCherry.90 beta

   Plasmid

   #108223

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 beta

   Depositor

   Didier Picard

   Article

   Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

   Insert

   mCherry-Hsp90b (HSP90AB1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

   Plasmid

   #194171

   Purpose

   Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-Associated Protein Tau 0N4R
   

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PD-1-miSFIT-Ce67

   Plasmid

   #124680

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCGN-MEF2B

   Plasmid

   #32959

   Purpose

   For mammalian expression of human MEF2B with an HA tag

   Depositor

   Ron Prywes

   Article

   Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

   Insert

   MEF2B (MEF2B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-43: GJA1-mEGFP

   Plasmid

   #109120

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

   Depositor

   Allen Institute for Cell Science

   Insert

   GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3/td5StayGold(c4)=GianCreg

   Plasmid

   #212022

   Purpose

   For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GianCreg (GOLGB1 Human)
   

   Tags

   td5StayGold(c4)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-EML4-ALK variant 3a L1196M/G1202R

   Plasmid

   #183831

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   L1196M/G1202R

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Promoter

   lac

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK262 (RAD21-mAC Neo)

    Plasmid

    #140538

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 WT B-Myb

    Plasmid

    #25965

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (MYBL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EML4-ALK variant 3a I1171N

    Plasmid

    #183832

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-Cdk9

    Plasmid

    #28102

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. p4xSTAT6-Luc2P

    Plasmid

    #35554

    Depositor

    Axel Nohturfft

    Insert

    4xStat6 promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    4xStat6

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPHK1 gRNA (BRDN0001147454)

    Plasmid

    #76813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT_TO_FlagHA_USP10

    Plasmid

    #127102

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    USP10 (USP10 Human)
    

    Expression

    Mammalian

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF1001

    Plasmid

    #143253

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    REST (REST Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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