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Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW213J116-Amp32C
Plasmid#78648PurposepSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ116-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR
Plasmid#61778PurposeLuciferase reporter containing the 3'UTR of mouse YwhagDepositorInsertmouse Ywhag 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3A
Plasmid#79501PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDSDepositorInsertWNT3A (WNT3A Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKalpha RP deletion
Plasmid#33328DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
UseTagsFLAGExpressionMammalianMutationDeleted RP domain (amino acids 167-189 missing)PromoterSV40Available sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-deltaNLS1
Plasmid#22153DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationdeletion of amino acids 297-304PromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR
Plasmid#61779PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1DepositorInsertmouse Xbp1 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat 1-460 K193A
Plasmid#33326DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationtruncated at 460, K193A (S3V, G96R, and P108L als…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat 1-460 D311A
Plasmid#33325DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationtruncated at 460, D311A (S3V, G96R, and P108L als…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
p201N 1514
Plasmid#47025PurposeContains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencingDepositorInsertsgma-miR1514 recognition sequence
nptII selectable marker
UseRNAiTagsExpressionMutationPromoterGmUbi and StUbi-3Available sinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
GUCY1B3
Plasmid#39121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationThis is a complex of two proteins; GUCY1A3 (aa 46…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS1044
Plasmid#63797PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection markerDepositorInsert3xFLAG followed by KlLEU2
UseE. coli cloning vectorTags3xFLAGExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Paxillin-22
Plasmid#57849PurposeLocalization: Focal Contacts, Excitation: 548, Emission: 561DepositorInsertPaxillin
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCplac111-KOG1 (pAN58)
Plasmid#39209DepositorInsertKOG1 (KOG1 Budding Yeast)
UseTagsmycExpressionYeastMutationL1512PPromoterKOG1Available sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNH002
Plasmid#42145DepositorInsertN-TAP TAG::[(G4S)3]::mTFP1
UseTagsTAP TAGExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH026
Plasmid#42148DepositorInsertmTFP1::linker::TAP TAG
UseTagsTAP-TagExpressionMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH001
Plasmid#42144DepositorInsertTAP-tag
UseTagsTAP-tagExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only