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Plasmid#163063PurposeEntry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)DepositorInsertCheRiff
UseGateway entry vectorTagseGFPExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRbm10/Cre
Plasmid#89648PurposeExpresses an Rbm10-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Rbm10 (Rbm10 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB30
Plasmid#122247PurposeLentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_LamB1
Plasmid#181999PurposeCMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell linesDepositorInsertSTf-LamB1
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSmad4/Cre
Plasmid#89651PurposeExpresses a Smad4-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Smad4 (Smad4 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE390
Plasmid#149280PurposeT-DNA encoding TRV2 with gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x3
Plasmid#58773PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pED7x26
Plasmid#134813PurposeStringent phage propagation reporter containing pIII-16-29-34-TAGADepositorInsertpIII 16-TAGA 29-TAGA 34-TAGA
UseSynthetic BiologyTagsExpressionBacterialMutation16-TAGA 29-TAGA 34-TAGAPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE387
Plasmid#149285PurposeT-DNA encoding TRV2 with FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_FT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ138A
Plasmid#69280PurposeGolden Gate entry vector; 8th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ137A
Plasmid#69279PurposeGolden Gate entry vector;7th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG418R-GMRWhite
Plasmid#165883PurposeG418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU205
Plasmid#167685PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU321
Plasmid#167681PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::asCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU320
Plasmid#167682PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_sgRNA
Plasmid#68422PurposeTransient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertGluc sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNeo3/Cre
Plasmid#89653PurposeExpresses a Neomycin-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Neomycin
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ158
Plasmid#69307PurposeGateway entry vector with hSpCas9 (Human codon optimized)DepositorInserthSpCas9 (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationNLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A8
Plasmid#99882PurposeRecipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.DepositorTypeEmpty backboneUseTALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-IVS-Syn21-GFP-P10
Plasmid#104614PurposeExpresses GFP upon binding of TALE3 to VAS3DepositorInsert3xVAS3
UseTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-IVS-Syn21-GFP-P10
Plasmid#104613PurposeExpresses GFP upon binding of TALE1 to VAS1DepositorInsert3xVAS1
UseTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CT611
Plasmid#233669PurposeRed light-inducible Cre-CDepositorInsertCre-230C-FHY1
UseCre/LoxTagsExpressionMammalianMutationNonePromoterCAGGAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
IK331
Plasmid#233653PurposeDox-inducible blue light-inducible Cre-NDepositorInsertCre-N229-pmag
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
IK332
Plasmid#233654PurposeDox-inducible blue light-inducible Cre-CDepositorInsertCre-230C-nmag
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_BasA3G
Plasmid#225145PurposeGateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editingDepositorInsertsBasA3G-zCas9(D10A)-UGI
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UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_EtA3C
Plasmid#225146PurposeGateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editingDepositorInsertEtA3C-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_DlA3G
Plasmid#225148PurposeGateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editingDepositorInsertDlA3G-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_LoA3GX1
Plasmid#225149PurposeGateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editingDepositorInsertLoA3GX1-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
act5C-mScarlet
Plasmid#230906PurposeUbiquitous expression of mScarlet in Drosophila melanogasterDepositorInsertmScarlet
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
UAST-uMCas12a+nls2x
Plasmid#230909Purposeinducible expression of Cas12a+ with Gal4.DepositorInsertCas12a+
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGRACE
Plasmid#230910PurposeTracing of CRISPR activity in whole animals.DepositorInsertGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
LAG3-DEM (L3#1)
Plasmid#227739PurposeDesigner Epigenome Modifier (DEM) designed to target the LAG3-promoterDepositorInsertDesigner epigenome modifier targeting LAG3-promoter (Homo sapiens)
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
apt-CBE-SL3
Plasmid#219940PurposeMammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP markerDepositorInsertMCP_16-aa_evo-rA1_2xUGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST P38KTRDronpa
Plasmid#205763PurposeExpression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)DepositorInsertP38 KTR Dronpa
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST ERKKTRrsFastLime
Plasmid#205765PurposeExpression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)DepositorInsertERK KTR rsFastLime
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCF.111
Plasmid#227187PurposepORTMAGE-Ec1 + supplement E. coli SSBDepositorInsertCspRecT, mutL_E32K, E. coli SSB
UseTagsExpressionBacterialMutationmutL E32KPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC293-P4B1
Plasmid#203134PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293DepositorInsertEGFP/BC293
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC170-P2G2
Plasmid#203011PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC170DepositorInsertEGFP/BC170
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC157-P2F1
Plasmid#202998PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC157DepositorInsertEGFP/BC157
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC120-P2B12
Plasmid#202961PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC120DepositorInsertEGFP/BC120
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC121-P2C1
Plasmid#202962PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC121DepositorInsertEGFP/BC121
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC282-P3H6
Plasmid#203123PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC282DepositorInsertEGFP/BC282
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC232-P3D4
Plasmid#203073PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC232DepositorInsertEGFP/BC232
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only