We narrowed to 49,922 results for: des.1

1. mCherry_eDHFR_SIM#1

   Plasmid

   #164646

   Purpose

   tag SIM with mCherry and eDHFR

   Depositor

   Michael Lampson, Huaiying Zhang

   Article

   Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

   Insert

   SIM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiGuide_GFP_sgEPAS1#1

   Plasmid

   #183119

   Purpose

   pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.shNkx2-1

   Plasmid

   #32400

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   shRNA targetting Nkx2-1 (Nkx2-1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pGFPmAID_htz-1

   Plasmid

   #174214

   Purpose

   Donor plasmid for tagging htz-1 with GFP::mAID

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Insert

   GFP::mAID::htz-1 tagging donor
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP1297‐1

   Plasmid

   #87247

   Purpose

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

   Insert

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
   

   Use

   Tags

   gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACCS3-1

   Plasmid

   #78559

   Purpose

   4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.

   Depositor

   James Kaper

   Article

   Giron et al Vaccine. 1995 Jul;13(10):939-46.

   Insert

   cs3 operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. shCDK6(1)

   Plasmid

   #73552

   Purpose

   shRNA(1) against CDK6

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

8. CpX-1

   Plasmid

   #218676

   Purpose

   Expresses complexin-1 in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   Rat complexin-1 (Cplx1 Rat)
   

   Use

   Tags

   6 his

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Interface-1 (a)

   Plasmid

   #204428

   Purpose

   Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

   Depositor

   Keir Neuman

   Article

   Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

   Insert

   hOpa1 isoform 1 (OPA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R627A K663A R683A

   Promoter

   CMV

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control-1

    Plasmid

    #204442

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L243A

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK73_htz-1

    Plasmid

    #174546

    Purpose

    htz-1 targeting gRNA expression

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Insert

    htz-1 targeting gRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Ce-U6 promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP1298‐1

    Plasmid

    #87248

    Purpose

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP553‐1

    Plasmid

    #87240

    Purpose

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. shCDK4(1)

    Plasmid

    #73550

    Purpose

    shRNA(1) against CDK4

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pTrichoGate-1

    Plasmid

    #160701

    Purpose

    derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    LacZ bordered by BsaI restrction sites

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. peCALWY-1

    Plasmid

    #22233

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    linker consists of 9 GGSGGS repeats

    Promoter

    Available since

    Nov. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMiNGo11-1

    Plasmid

    #126056

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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