161,123 results

1. pCMV-dCas12a-A3A

   Plasmid

   #193638

   Purpose

   Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse b8 full length

   Plasmid

   #217817

   Purpose

   Full length mouse integrin b8 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB8 (Itgb8 Mouse)
   

   Use

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC- AVITAG-CTH10STII- -GG

   Plasmid

   #215805

   Purpose

   Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. HRH3-DuET

   Plasmid

   #213314

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HRH3 (HRH3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-mCherry(-6)

   Plasmid

   #205045

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   mCherry(-6)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2636

   Plasmid

   #142343

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFDP2 (TFDP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0940

   Plasmid

   #141761

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF639 (ZNF639 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0810

   Plasmid

   #141714

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOHLH1 (SOHLH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ZNF846

   Plasmid

   #88355

   Purpose

   Donor Vector containing ZNF846 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF846 (ZNF846 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-60 roGFP2-Orp1-His

    Plasmid

    #64976

    Purpose

    Bacterial expression of His-tagged roGFP2-Orp1

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cys-60nmSAH-SPYcatcher

    Plasmid

    #214840

    Purpose

    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-60nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-CTCF

    Plasmid

    #116728

    Purpose

    Lentiviral expression of CTCF

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CTCF (CTCF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKvenus-Scribble

    Plasmid

    #58738

    Purpose

    Expresses Venus-tagged human Scribble protein

    Depositor

    Ian Macara

    Article

    Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-wtTnsAB

    Plasmid

    #234731

    Purpose

    Wild-type human-codon optimized PseTnsAB in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-wtTnsC

    Plasmid

    #234732

    Purpose

    Wild-type human-codon optimized PseTnsC in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    AURKA minimal

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 His-V5 Myc

    Plasmid

    #176049

    Purpose

    Expresses Myc tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNCS-Antares

    Plasmid

    #74279

    Purpose

    Constitutive expression of Antares in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Antares
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJL 89

    Plasmid

    #98558

    Purpose

    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Enh 35S

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-mCherry-Rheb

    Plasmid

    #192436

    Purpose

    Encodes mCherry-tagged wild-type Rheb.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-PGK-Neo-PIP-FUCCI

    Plasmid

    #118616

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. MSCV h c-MYC IRES GFP

    Plasmid

    #18119

    Depositor

    John Cleveland

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    The CUG which is the start site for Myc I is pres…

    Promoter

    Available since

    June 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. NLS-mCherry-LEXY (pDN122)

    Plasmid

    #72655

    Purpose

    Blue light-inducible nuclear export of mCherry

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    NLS-mCherry-LEXY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOpen-GBDpol (exo)

    Plasmid

    #165572

    Purpose

    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pAAV INS2x mRFP-3MREs (AAV-beta)

    Plasmid

    #206928

    Purpose

    Beta cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Article

    Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INSx2

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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