165,187 results

1. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-KLF5

   Plasmid

   #40918

   Depositor

   Jin-Tang Dong

   Article

   Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

   Insert

   Kruppel-like factor 5 (KLF5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. Integrin beta 2 - mYFP

   Plasmid

   #8638

   Depositor

   Timothy Springer

   Article

   Kim et al Science 2003 Sep 19;301(5640):1720-5.

   Insert

   Integrin beta 2 (ITGB2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   stop codon of beta2 was replaced with AgeI restri…

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-sfGFP

   Plasmid

   #102634

   Purpose

   In vitro expression of sfGFP from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   sfGFP
   

   Tags

   strep tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbA6c-RFP

   Plasmid

   #35290

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAdx-CMV-copGFP

   Plasmid

   #73346

   Purpose

   Express copGFP under CMV promoter

   Depositor

   Kazuhiro Oka

   Insert

   CMV-copGFP
   

   Use

   Adenoviral

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-MTS-EGFP

   Plasmid

   #190270

   Purpose

   Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

   Depositor

   Christian Münch

   Article

   Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

   Insert

   MTS-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGKp-GFP-YAP

   Plasmid

   #174172

   Purpose

   Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRL093 pattB_exorh:EGFP_MCS

    Plasmid

    #213514

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKAR2-Br512

    Plasmid

    #161875

    Purpose

    Expresses Br512 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Maranhao et al medRxiv

    Insert

    Modified DNA Polymerase I Large Fragment (Br512)
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Promoter

    T7, tetPA

    Available Since

    Nov. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-TRE-NpHR-EYFP

    Plasmid

    #171621

    Purpose

    TRE-NpHR-EYFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-NpHR-EYFP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AICSDP-9:DSP-mEGFP

    Plasmid

    #87424

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    DSP Homology Arms with linker-mEGFP (DSP Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-MCS UBC promoter

    Plasmid

    #128045

    Purpose

    Lentiviral vector with UBC promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-Etv6

    Plasmid

    #131599

    Purpose

    expression of mouse Etv6 in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Etv6 (Etv6 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 HA E2F3A

    Plasmid

    #236437

    Purpose

    transient overexpression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. aeBlue chromoprotein

    Plasmid

    #117846

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, aeBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. GPR158-Tango

    Plasmid

    #66332

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR158 (GPR158 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-NLS-mCherry

    Plasmid

    #231890

    Purpose

    mCherry-tagged nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    mCherry-SV40 NLS-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLminP_Luc2P_RE55

    Plasmid

    #90400

    Purpose

    C-REL - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    CREL

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Expression

    Insect

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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