We narrowed to 38,424 results for: ANT
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Plasmid#213261PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR120 (FFAR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.A184S
Plasmid#106201PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3
Plasmid#106204PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184S + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mouse b5 full length
Plasmid#217815PurposeFull length mouse integrin b5 expressionDepositorInsertITGB5 (Itgb5 Mouse)
UseTagsP2A-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR1D-DuET
Plasmid#213318PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR1D (HTR1D Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S
Plasmid#106177PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
HTR1B-DuET
Plasmid#213317PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR1B (HTR1B Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-STU
Plasmid#138110PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminatorDepositorInsertLbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v1 IQSEC1 WT
Plasmid#162016PurposeExpression of IQSEC1 splice variant 1DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v2 IQSEC1 WT
Plasmid#162017PurposeExpression of IQSEC1 splice variant 2DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax SpG
Plasmid#187895PurposeAll-in-one prime editor piggyBac transposon with PEmax, SpG variantDepositorInsertSpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BM00369
Plasmid#155338PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GABBR1-DuET
Plasmid#213247PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGABBR1 (GABBR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ab
Plasmid#110799PurposeExpression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertantibody against mouse interferon alpha
UseTagsER signal peptide, His6 tag, and myc epitope tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v3 IQSEC1 WT
Plasmid#162018PurposeExpression of IQSEC1 splice variant 3DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-GFP-v4 IQSEC1 WT
Plasmid#162019PurposeExpression of IQSEC1 splice variant 4DepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6 (-cyt)
Plasmid#137817Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region, R296K (please see dep…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3
Plasmid#106206PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72A + mRuby3 fusionPromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsHis8ExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALCRb-DuET
Plasmid#213193PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCALCRb (CALCRL Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.A184V
Plasmid#106202PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
human a5 full length
Plasmid#217818PurposeFull length human integrin a5 expressionDepositorInsertITGA5 (ITGA5 Human)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M1_1-pTRNA-scf 2.1 (GB2603)
Plasmid#160568PurposetRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertM1_1-pTRNA-scf 2.1
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-ARF6-T157N-TagRFP
Plasmid#162030PurposeFast cycling ARF mutantDepositorInsertARF6 (ARF6 Human)
UseLentiviralTagsExpressionMutationT151NPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ADRB2-DuET
Plasmid#213181PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRB2 (ADRB2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPRC6A-DuET
Plasmid#213299PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPRC6A (GPRC6A Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRA2A-DuET
Plasmid#213177PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRA2A (ADRA2A Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.S72A
Plasmid#106203PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.A184S
Plasmid#106195PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterGFAPAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpG
Plasmid#179081PurposeAll-in-one prime editor piggyBac transposon, SpG variantDepositorInsertSpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135L, S1136W, G1218K, E1219Q, R1335Q, T1337RPromoterCAGAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-N289A-H290A
Plasmid#82032PurposePlasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationEndonuclease-dead mutant: N289A+H290A CtIP; Mutat…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP-pp4640
Plasmid#191846PurposeExpresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusionDepositorInsertmEGFP-pp4640
UseTagsmEGFPExpressionMammalianMutationmEGFP fusion in Nterminal, some silent point muta…PromoterCMVAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO11-Jeff-pcDNA3.1-
Plasmid#52503Purposeexpresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO11 (FBXO11 Human)
UseTagsFLAG-HAExpressionMammalianMutationQ491L (Jeff)PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v3 (-cyt)
Plasmid#137813Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v3 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region, N280D in the V3 regionPromoterPGKAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A
Plasmid#106179PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterhSynapsinAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PE NG
Plasmid#174554PurposeAll-in-one prime editor piggyBac transposon, NG variantDepositorInsertSpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationL1111R, G1218R, E1219F, A1322R, R1335V, T1337RPromoterCAGAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only