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Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC1125
Plasmid#41561DepositorInsertUra3-GAL1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
perbB2K732M-ECFP
Plasmid#40269DepositorInserterbB2 (ERBB2 Human)
UseTagsecfpExpressionMammalianMutationK732MPromoterCMVAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-Sox17
Plasmid#60669PurposePiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoterDepositorInsertSox17 (Sox17 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103_GB
Plasmid#65991PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23107_GB
Plasmid#65999PurposeMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23116_GB
Plasmid#66003PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD
Plasmid#133300PurposeThe puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeTagsExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-UTRN-mini
Plasmid#158749PurposeExpresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cellsDepositorInsertUTRN-mini
UseTagseGFPExpressionMammalianMutationPromoterCMV IE94Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuroATT
Plasmid#47539PurposeRetroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSA120
Plasmid#59789PurposeC. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operonDepositorInsertshsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Tal1
Plasmid#60670PurposePiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoterDepositorInsertTal1 (Tal1 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1)
Plasmid#80049PurposeExpression of EPN-01 mutated in TSG101 binding site.DepositorInsertEPN-01(deltaPTAP)
UseTagsMycExpressionMammalianMutationPTAP10AAAAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSY14-ptb
Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEB2-mNeptune2
Plasmid#104012PurposePlasmid encoding mNeptune2DepositorInsertmNeptune2
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… R32…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
UseTagsExpressionBacterialMutationPromoterPcphy23Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR2
Plasmid#59012PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR2 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-(935-1026)-NES-mut
Plasmid#42021DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa935-1026, F951A, F955A, I958A, F960APromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
KG#65
Plasmid#110933PurposeExpresses proteins in cholinergic neurons in the ventral nerve cord of C. elegansDepositorInsertsunc-17 beta promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoV-NSP3-c001
Plasmid#173082PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep
UseTagsmCeruleanExpressionBacterialMutationE1000-K1173, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCaSpeR4 mito roGFP2-Orp1
Plasmid#65001PurposeExpression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1*
Plasmid#104000PurposePlasmid encoding mRFP1*DepositorInsertmRFP1*
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLAVIKEF...T…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1
Plasmid#104001PurposePlasmid encoding mRFP1DepositorInsertmRFP1
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC1136
Plasmid#41562DepositorInsertUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T-
Plasmid#161487PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT206/Sept7shRNA
Plasmid#38299DepositorInsertSeptin 7 (Septin7 Mouse)
UseTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-EGFP
Plasmid#102782PurposeBaculovirus rescue vector, VSVGED pseudotype, eGFPDepositorInsertEGFP
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationPromoterCMVAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BpBBS
Plasmid#199467PurposeArabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.DepositorInsertBpBBS
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Pitx2
Plasmid#60668PurposePiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoterDepositorInsertPitx2 (Pitx2 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
phNFL-Q332P
Plasmid#132599PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutationDepositorInsertNefl (Nefl Human)
UseTagsExpressionMammalianMutation995A>C;Gln332ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-Q333P-EGFP
Plasmid#132602PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation998A>C, 999G>C; Gln333ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Orp1
Plasmid#64997Purposefor expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationmitochondrial targeting sequencePromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXY1-spc-ptb
Plasmid#90489PurposepXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
phNFL-E396K
Plasmid#132595PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1186G>A; Glu396LysPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only