19,640 results

1. pBR-Lent-STAT5A-CA

   Plasmid

   #170662

   Purpose

   Lentiviral expression of constitutively active STAT5A.

   Depositor

   Alexander Ruthenburg

   Article

   Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

   Insert

   STAT5A (STAT5A Human)
   

   Use

   Lentiviral

   Mutation

   H298R and S710F

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS316-RGR-GFP

   Plasmid

   #51056

   Purpose

   Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. mscv2.2-NAIP5

   Plasmid

   #60205

   Purpose

   Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

   Depositor

   Russell Vance

   Article

   Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

   Insert

   NAIP5
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHACK-GAL4>QF2

   Plasmid

   #80275

   Purpose

   GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEGFP-WT-STAT3

   Plasmid

   #71450

   Purpose

   Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Green Lindoblum

   Plasmid

   #163113

   Purpose

   Expresses biosensor for lactate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Lindolum
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-HisD-miniGFP1

    Plasmid

    #188896

    Purpose

    Express 6xHis-Tag miniGFP1 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XFlagNICD2

    Plasmid

    #20184

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 2 intracellular domain (Notch2 Mouse)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 2 Intracellular Domain (Met…

    Promoter

    CMV

    Available Since

    Jan. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Tight-Puro_Sox10

    Plasmid

    #46536

    Purpose

    A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Sox10 (Sox10 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-puro

    Plasmid

    #17923

    Purpose

    Insect expression vector

    Depositor

    David Sabatini

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Drosophila metallothionein gene promoter drives e…

    Expression

    Insect

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid

    #182306

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    TRE3G-Bi-directional

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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