Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,082 results for: CHL

Clear keywords
Showing: 10801 - 10850 of 11082 results

  6. pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

    Plasmid
    #186414
    Purpose
    AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Promoter
    Basal promoter of the FOG gene
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186414-plasmid-map-sequence-id-369043

    Enlarge

  16. pCCM’ CbbQ-

    Plasmid
    #162711
    Purpose
    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    Inactivation of CbbQ subunit of CbbOQ rubisco act…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162711-plasmid-map-sequence-id-324197

    Enlarge

  17. pCCM’ ΔacRAF

    Plasmid
    #162710
    Purpose
    Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    pCCM' with a deletion of putative rubisco ch…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162710-plasmid-map-sequence-id-324185

    Enlarge

  21. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

    Enlarge

  22. pSR357.12

    Plasmid
    #125107
    Purpose
    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115
    Depositor
    Jeffrey Tabor
    Article
    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d
    Inserts
    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    A84T - see depositor comments below and Q183K-see…
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125107-plasmid-map-sequence-id-247775

    Enlarge

  29. pTSlb-R756S

    Plasmid
    #60730
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Jan. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60730-plasmid-map-sequence-id-97716

    Enlarge

  30. pTSara-R756S

    Plasmid
    #60725
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60725-plasmid-map-sequence-id-97713

    Enlarge

  31. pTSara-Q758C

    Plasmid
    #60724
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60724-plasmid-map-sequence-id-97710

    Enlarge

  34. pJ175

    Plasmid
    #29493
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. pJ158

    Plasmid
    #29492
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. pJ177

    Plasmid
    #29494
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  37. pJ178

    Plasmid
    #29495
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  38. pJ139

    Plasmid
    #29489
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  39. pJ143

    Plasmid
    #29490
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  40. pJ138

    Plasmid
    #29488
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  44. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

    Enlarge

  45. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

    Enlarge

  47. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

    Enlarge

Showing: 10801 - 10850 of 11082 results