We narrowed to 10,805 results for: CHL

1. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

   Plasmid

   #185447

   Purpose

   Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Inserts

   TRF1 (TERF1 Human)
   POT1 (POT1 Human)
   TPP1 (TPP1 Human)
   TIN2 (TINF2 Human)
   

   Use

   Tags

   MBP, YBBR, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

   Plasmid

   #223065

   Purpose

   Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Three regions of SpCas9 encode type IIS restricti…

   Promoter

   Dual T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Cavbeta1 Ca2+ channel [N7/18R-rat IgG2a] - Chimeric

   Plasmid

   #231836

   Purpose

   Mammalian expression plasmid of anti-Cavbeta1 Ca2+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N7/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Cavbeta1 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNB1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAP1

   Plasmid

   #63674

   Purpose

   Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

   Depositor

   Nicola Patron

   Article

   Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

   Insert

   RFP cloning selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

   Plasmid

   #111903

   Purpose

   Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

   Depositor

   Andrew Carter

   Inserts

   His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
   DYNC1I2 (DYNC1I2 Human)
   DYNC1LI2 (DYNC1LI2 Human)
   DYNLT1 (DYNLT1 Human)
   DYNLRB1 (DYNLRB1 Human)
   DYNLL1 (DYNLL1 Human)
   

   Use

   Tags

   8xHis, SNAPf-tag, TEV site, and ZZ-tag

   Expression

   Insect

   Mutation

   Optimised for expression in Sf9 cells.

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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