We narrowed to 24,612 results for: Nes

1. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

   Plasmid

   #215901

   Purpose

   Expression of TNRC6A-CIM1 domain with S1346A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   contain aa1224-1528, S1346A

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-BOS-LGP2-Myc

   Plasmid

   #215874

   Purpose

   LGP2-Myc expression vector for overexpression

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

   Insert

   LGP2 (DHX58 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. VF-Sensor

   Plasmid

   #208927

   Purpose

   in vitro RSK-Sensor for luminescent detection of RSK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   SOS1 (SOS1 Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDE4B-Sensor

   Plasmid

   #208929

   Purpose

   in vitro JNK-Sensor for luminescent detection of JNK-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
   

   Use

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_tKiMBImut-T2A-caMEK

   Plasmid

   #199579

   Purpose

   Express tKiMBImut(AA) and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
   constitutively active MEK
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGA-red-mini

   Plasmid

   #196338

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGA-red-maxi

   Plasmid

   #196337

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGA-blue

   Plasmid

   #196336

   Purpose

   Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tet pLKO.1-shCLTC v2 puro

   Plasmid

   #192348

   Purpose

   Lentiviral expression vector for an inducible shCLTC v2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   shCLTC v2 (CLTC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK 3xFLAG-CSE1L neo

    Plasmid

    #192341

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. tet pLKO.1-shCse1l v1 puro

    Plasmid

    #192342

    Purpose

    Lentiviral expression vector for an inducible shCse1L v1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shCse1l v1 (Cse1l Budding Yeast)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet pLKO.1-shCLTC v1 puro

    Plasmid

    #192347

    Purpose

    Lentiviral expression vector for an inducible shCLTC v1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    shCLTC v1 (CLTC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK 3xFLAG-DYNLL2 zeo

    Plasmid

    #192321

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-DYNLL2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    DYNLL2 (DYNLL2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN_TT 3xFLAG-BCAS2

    Plasmid

    #192294

    Purpose

    Gateway entry vector for an inducible 3xFLAG-BCAS2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BCAS2 (BCAS2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_TT 3xFLAG-DYNLL2

    Plasmid

    #192296

    Purpose

    Gateway entry vector for an inducible 3xFLAG-DYNLL2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    DYNLL2 (DYNLL2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

    Plasmid

    #199253

    Purpose

    Expression construct encoding the high specificity SpCas9-KA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1_B1ChR2_TS_mScarlet_ER

    Plasmid

    #195191

    Purpose

    Highly K+ selective channelrhodopsin

    Depositor

    Peter Hegemann, Johannes Vierock

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Bilabrum sp. K+ selective channelrhodopsin 2
    

    Use

    Tags

    ER-export sequence, Kir2.1 trafficking motif, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

    Plasmid

    #178986

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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