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Showing: 8681 - 8720 of 8781 results

  1. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  3. pCMV-BRAF-REG-T241P-Halo

    Plasmid
    #202549
    Purpose
    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.
    Depositor
    Deborah Morrison
    Article
    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e
    Insert
    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Threonine 241 mutated to proline
    Promoter
    CMV
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    202549-plasmid-map-sequence-id-401163

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  4. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

    Plasmid
    #120404
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Containing complete variable region with a mutati…
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120404-plasmid-map-sequence-id-232440

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  5. Fibronectin-human-mutated V2 O-Glycosylation site

    Plasmid
    #120405
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120405-plasmid-map-sequence-id-232458

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  9. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  10. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-490369

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  12. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  17. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

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  18. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

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  19. pcDNA3.1-GSDMD_29-484

    Plasmid
    #218231
    Purpose
    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-484
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218231-plasmid-map-sequence-id-436177

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  20. pcDNA3.1-GSDMD_29-270

    Plasmid
    #218232
    Purpose
    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218232-plasmid-map-sequence-id-430566

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  23. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid
    #173762
    Purpose
    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173762-plasmid-map-sequence-id-353460

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  24. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid
    #173763
    Purpose
    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173763-plasmid-map-sequence-id-353459

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  25. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid
    #173764
    Purpose
    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173764-plasmid-map-sequence-id-353458

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  29. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  31. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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  33. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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  35. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

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  36. pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)

    Plasmid
    #231234
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    TAD deleted (Δ1-143), deletion of degron 1 & …
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231234-plasmid-map-sequence-id-459167

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Showing: 8681 - 8720 of 8781 results