We narrowed to 11,223 results for: SHA

1. kank3_pJet1.2

   Plasmid

   #121981

   Purpose

   for generating kank3 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank3 (kank3 Zebrafish)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. kank4_pJet1.2

   Plasmid

   #121982

   Purpose

   for generating kank4 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank4 (kank4 Zebrafish)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

   Plasmid

   #110735

   Purpose

   Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   H2B-mOrange2
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECE M2-SNX17 S437A

   Plasmid

   #69812

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASt-SIK3 S563A

   Plasmid

   #52976

   Purpose

   insect expression of SIK3 S563A PKA phosphorylation defective mutant

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   S563A PKA phosphorylation defective

   Promoter

   Hsp70

   Available since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

6. USH1C_PDZ_1

   Plasmid

   #103936

   Purpose

   Protein expression and purification of P73 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   USH1C (USH1C Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pfast bac-flag Fbxl3

   Plasmid

   #51894

   Purpose

   Backbone plasmid is pfastBacHTa from invitrogen

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Fbxl3 (Fbxl21 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pGEMHE-Trim21-OLLAS

   Plasmid

   #237438

   Purpose

   Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

   Insert

   Trim21 (Trim21 Mouse)
   

   Use

   Tags

   OLLAS

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLuc-N-HiBiT

   Plasmid

   #207117

   Purpose

   Bacterially expressed HiBiT-FLuc for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   luciferin 4-monooxygenase
   

   Use

   Tags

   HiBiT Tag and His-tag with thrombin site

   Expression

   Bacterial

   Mutation

   Codon optmization for expression in BL21DE3

   Promoter

   T7 Promotor

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLuc-C-HiBiT

    Plasmid

    #207118

    Purpose

    Bacterially expressed FLuc-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    luciferin 4-monooxygenase
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLuc-C-NLuc

    Plasmid

    #234566

    Purpose

    Bacterially expressed FLuc-C-NLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Firefly luciferase
    

    Use

    Tags

    NanoLuciferase-thrombin site-10xHis and T7 gene 1…

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH47822_Myc-Sr62TK

    Plasmid

    #233536

    Purpose

    Expresses Myc-Sr62TK in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK
    

    Use

    Tags

    Myc tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

    Plasmid

    #234883

    Purpose

    non-targeting CRISPRi control

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6-CFAPoptim-KAN

    Plasmid

    #221128

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS415-TEF1pr-SEC63-FAPoptim

    Plasmid

    #221116

    Purpose

    FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim-SEC63
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDA_052_HygroR

    Plasmid

    #228937

    Purpose

    Plasmid for cloning of custom gRNA using EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS415-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221115

    Purpose

    FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-ImpBeta1-mEosEM

    Plasmid

    #218933

    Purpose

    Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    Importin Beta (KPNB1 Human)
    

    Use

    Tags

    6xHis and mEos4b (EosEM)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Start Stuffer TurboID V5 T2A BFP

    Plasmid

    #233257

    Purpose

    Expression of BFP-tagged vector for Turbo ID experiments

    Depositor

    David Bryant

    Article

    Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

    Insert

    TurboID V5 T2A BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS413-TEF1pr-ANP1-FAPoptim

    Plasmid

    #221120

    Purpose

    FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ANP1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS413-TEF1pr-ERG6-FAPoptim

    Plasmid

    #221121

    Purpose

    FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ERG6-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS413-TEF1pr-PMA1-FAPoptim

    Plasmid

    #221122

    Purpose

    FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    PMA1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS413-TEF1pr-VPH1-FAPoptim

    Plasmid

    #221125

    Purpose

    FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    VPH1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EHMT1_exon 3_gRNA

    Plasmid

    #228809

    Purpose

    CRISPR targeting of human EHMT1

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    gRNA targeting EHMT1 exon 3 (EHMT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP_EHMT1B1-3

    Plasmid

    #228811

    Purpose

    Expression of mouse EHMT1 with deletion of aa 1185-1298

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    EHMT1 (Ehmt1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 1185-1298

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP_EHMT1B1-4

    Plasmid

    #228812

    Purpose

    Expression of mouse EHMT1 with deletion of aa 809-1298

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    EHMT1 (Ehmt1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 809-1298, See Depositor Comments

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge