We narrowed to 4,072 results for: p2a

1. pCSIIbleo-iRFP670-P2A-EGFP

   Plasmid

   #198060

   Purpose

   The lentiviral vector for iRFP670 and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC-ZsGreen-P2A-Hygro

   Plasmid

   #124301

   Purpose

   Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-xFNLS-P2A-Puro

   Plasmid

   #110872

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-BE4GamRA-P2A-Puro

   Plasmid

   #112673

   Purpose

   Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE4GamRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pFastbacHTB HA-human PP2A Cα D88N

   Plasmid

   #132633

   Purpose

   expresses HA-human PP2A Cα D88N in Hi5 insect cell

   Depositor

   Wenqing Xu

   Article

   Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

   Insert

   PP2A Cα D88N
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ICP8-P2A/Crimson (pSLIK4)

   Plasmid

   #113859

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK433 (mAID-P2A-BSD)

   Plasmid

   #168228

   Purpose

   C-terminal tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

   Insert

   mAID-P2A-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AID-BE4max-P2A-GFP

   Plasmid

   #157948

   Purpose

   Mammalian expression of AID-BE4max-P2A-GFP

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   AID-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BlastR-P2A-DD-TCOF1-donor

    Plasmid

    #60748

    Purpose

    Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

    Depositor

    Benhur Lee

    Article

    Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. EGFP-P2A-APEX2-csChBP

    Plasmid

    #108877

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2-csChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-xBE4Gam-P2A-Puro

    Plasmid

    #112676

    Purpose

    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE4Gam
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4TO-24xGCN4_v1-P2A-sfGFP

    Plasmid

    #61057

    Purpose

    sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    24xGCN4_v1 and sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pAAV2-GCaMP5g-P2A-PAmCherry

    Plasmid

    #133411

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

    Plasmid

    #190134

    Purpose

    expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

    Depositor

    Joshua Knowles

    Article

    Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

    Insert

    eGFP-p2A-HA-GPR151(p.Arg95Ter)
    

    Use

    Lentiviral

    Tags

    HA, eGFP, and p2A

    Expression

    Mammalian

    Mutation

    p.Arg95Ter mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BE3-P2A-EGFP (pJUL977)

    Plasmid

    #123612

    Purpose

    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK377 (Hygro-P2A-mCherry2)

    Plasmid

    #121188

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mCherry2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. katushka-FMDV-P2A-pTRIP

    Plasmid

    #163522

    Purpose

    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

    Depositor

    Thomas Weber

    Insert

    stopless Katushka-P2A
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

    Plasmid

    #121542

    Purpose

    Chronos with red nucleus-targeted fluorescence for excitatory neurons

    Depositor

    Matthias Prigge

    Insert

    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CamKII

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRepair-MPP8_mAID-P2A-BFP

    Plasmid

    #175036

    Purpose

    Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    mAID-P2A-BFP
    

    Use

    Hdr donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJL2-mGold-P2A-EBFP2

    Plasmid

    #157998

    Purpose

    Co-expression of mGold and EBFP2 in mammalian cells.

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold-P2A-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold is mVenus with L46F;T63S mutations

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. MSCV-LMP2A-IRES-GFP

    Plasmid

    #220352

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-postASAP-p2a-ChrimsonR

    Plasmid

    #178794

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and ChrimsonR
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMK451 (BSD-P2A-dTAG)

    Plasmid

    #214381

    Purpose

    dTAG for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMKO.1-Puro-shPP2A-Aalpha

    Plasmid

    #15249

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pLenti-ABE7.10-P2A-Puro

    Plasmid

    #112674

    Purpose

    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

    Depositor

    Lukas Dow

    Insert

    ABE7.10
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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