Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 2,360 results for: EFS

Showing: 1051 - 1100 of 2360 results

  7. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

    Enlarge

  9. pL0_EF-H4

    Plasmid
    #202178
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    H4 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202178-plasmid-map-sequence-id-405693

    Enlarge

  10. pL0_EF-fcpA

    Plasmid
    #202164
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    fcpA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202164-plasmid-map-sequence-id-405675

    Enlarge

  13. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

    Enlarge

  15. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

    Enlarge

  16. pCSIIbsr-Raichu-Rab5/PM

    Plasmid
    #214819
    Purpose
    Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    Depositor
    Michiyuki Matsuda
    Article
    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:
    Insert
    A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    214819-plasmid-map-sequence-id-427357

    Enlarge

  17. pL0_EF-Fld

    Plasmid
    #202181
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    202181-plasmid-map-sequence-id-421657

    Enlarge

  18. pL0_EF-P49

    Plasmid
    #202176
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    P49 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202176-plasmid-map-sequence-id-410914

    Enlarge

  20. pL0_EF-glna

    Plasmid
    #202183
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    glna terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202183-plasmid-map-sequence-id-405699

    Enlarge

  21. pL0_EF-act1_tr

    Plasmid
    #202171
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    act1 truncated terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202171-plasmid-map-sequence-id-405685

    Enlarge

  22. pL0_EF-epyC

    Plasmid
    #202185
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202185-plasmid-map-sequence-id-405701

    Enlarge

  23. pL0_EF-acc2

    Plasmid
    #202184
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    acc2 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202184-plasmid-map-sequence-id-405700

    Enlarge

  24. pL0_EF-dph1

    Plasmid
    #202182
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    dph1 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202182-plasmid-map-sequence-id-405698

    Enlarge

  25. pL0_EF-NR

    Plasmid
    #202179
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    NR terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202179-plasmid-map-sequence-id-405694

    Enlarge

  26. pL0_EF-fcpB

    Plasmid
    #202177
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    fcpB terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202177-plasmid-map-sequence-id-405692

    Enlarge

  27. pL0_EF-GPI1

    Plasmid
    #202175
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GPI1 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202175-plasmid-map-sequence-id-405691

    Enlarge

  28. pL0_EF-vapol

    Plasmid
    #202174
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    vapol terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202174-plasmid-map-sequence-id-405690

    Enlarge

  29. pL0_EF-lysA

    Plasmid
    #202173
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    lysA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202173-plasmid-map-sequence-id-405688

    Enlarge

  30. pL0_EF-HO

    Plasmid
    #202170
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    HO terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202170-plasmid-map-sequence-id-405684

    Enlarge

  31. pL0_EF-NRT

    Plasmid
    #202169
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54101 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    NRT terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202169-plasmid-map-sequence-id-405681

    Enlarge

  32. pL0_EF-sit4

    Plasmid
    #202168
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 48707 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    sit4 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202168-plasmid-map-sequence-id-405680

    Enlarge

  33. pL0_EF-CaMK2

    Plasmid
    #202167
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 39236 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    CaMK2 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202167-plasmid-map-sequence-id-405678

    Enlarge

  34. pL0_EF-fbaC5

    Plasmid
    #202166
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 41423 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    fbaC5 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202166-plasmid-map-sequence-id-405677

    Enlarge

  35. pL0_EF-OEE3

    Plasmid
    #202165
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54499 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    OEE3 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202165-plasmid-map-sequence-id-405676

    Enlarge

  36. pL0_EF-27877

    Plasmid
    #202162
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 27877 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    27877 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202162-plasmid-map-sequence-id-405674

    Enlarge

  37. pL0_EF-51183

    Plasmid
    #202161
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 51183 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202161-plasmid-map-sequence-id-405626

    Enlarge

  38. pL0_EF-GEF

    Plasmid
    #202160
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 41365 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GEF terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202160-plasmid-map-sequence-id-405625

    Enlarge

  39. pL0_EF-47740

    Plasmid
    #202159
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 47740 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    47740 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202159-plasmid-map-sequence-id-405624

    Enlarge

  40. pL0_EF-BCA

    Plasmid
    #202158
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 51305 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    BCA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202158-plasmid-map-sequence-id-405623

    Enlarge

  43. pVITRO-Fab_Theo-LUCID

    Plasmid
    #119048
    Purpose
    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119048-plasmid-map-sequence-id-231811

    Enlarge

  44. pVITRO-Fab_Quin-LUCID

    Plasmid
    #119049
    Purpose
    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119049-plasmid-map-sequence-id-231810

    Enlarge

  45. pVITRO-Fab_MTX-LUCID

    Plasmid
    #119047
    Purpose
    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2
    Inserts
    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    Use
    Tags
    FLAG-tag, His-tag, and Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α + CMV/SV40 enhancers and IRES
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119047-plasmid-map-sequence-id-231808

    Enlarge

Showing: 1051 - 1100 of 2360 results