We narrowed to 1,177 results for: POLI

1. pDECKO_GFP_AAVS1

   Plasmid

   #141211

   Purpose

   pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDECKO_mCherry_AAVS1_1801

   Plasmid

   #159090

   Purpose

   targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSPL3-hRPH3A_c.444Tmut

   Plasmid

   #190477

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.444G>T

   Promoter

   SV40

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSPL3-hRPH3A_c.1853Awt

   Plasmid

   #190478

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107578

   Purpose

   B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   fbp promoter Bacillus megaterium DSM319

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

   Plasmid

   #67991

   Purpose

   CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid

    #61365

    Purpose

    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D16A, D587A, H588A, N611A in Nm Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72667

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Human 3' HP1a AID GFP PuroR

    Plasmid

    #127906

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    HP1 (CBX5 Mustard Weed, Human)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

    Plasmid

    #67976

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

    Plasmid

    #67981

    Purpose

    Cas9 activity reporter (control) with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107582

    Purpose

    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fba promoter Bacillus megaterium DSM319

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL3E-hGPR56 e1m promoter normal

    Plasmid

    #52298

    Purpose

    Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107577

    Purpose

    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    gapdh promoter - B. megaterium DSM319

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid

    #52299

    Purpose

    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    15-bp deletion in the promoter

    Promoter

    Human GPR56 e1m promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RALA V25L

    Plasmid

    #122927

    Purpose

    Bacterial expression of RALA V25L

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    V25L

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RALA V25M

    Plasmid

    #122928

    Purpose

    Bacterial expression of RALA V25M

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    V25M

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RALA D130G

    Plasmid

    #122929

    Purpose

    Bacterial expression of RALA D130G

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D130G

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RALA S157A

    Plasmid

    #122930

    Purpose

    Bacterial expression of RALA S157A

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S157A

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RALA R176X

    Plasmid

    #122931

    Purpose

    Bacterial expression of RALA R176X

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R176X

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RALA WT

    Plasmid

    #122925

    Purpose

    Bacterial expression of RALA WT

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RALA G23D

    Plasmid

    #122926

    Purpose

    Bacterial expression of RALA G23D

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G23D

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SARS-CoV-2 S HexaPro

    Plasmid

    #154754

    Purpose

    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

    Depositor

    Jason McLellan

    Article

    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

    Insert

    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pCMV-Sport6-CD81-pHuji

    Plasmid

    #130904

    Purpose

    Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHuji (CD81 Human, Synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Leu-49 and Gly-50 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 synthetic, Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMV-Sport6-CD81-pHluorin

    Plasmid

    #130903

    Purpose

    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD81-pHluorin (CD81 aequorea victoria, Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Leu-49 and Gly-50 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV-Sport6-CD9-pHuji

    Plasmid

    #130906

    Purpose

    Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHuji (CD9 Human, Synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Asn50 and Asn51 in extrace…

    Promoter

    CMV

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid

    #61362

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHIS-MDH1-HIS

    Plasmid

    #184558

    Purpose

    Bacterial expression of human MDH1 with HIS Tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Malate dehydrogenase 1 (MDH1 Human)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid

    #61359

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid

    #61363

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid

    #61361

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge