We narrowed to 13,711 results for: cas9

1. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP791_PB_dCas9-SSSavi_BirA

   Plasmid

   #211786

   Purpose

   PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pCAGG abd pEF1a

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPRx-Sth1Cas9-L5

   Plasmid

   #140993

   Purpose

   Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

   Depositor

   Wilbert Bitter

   Article

   Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

   Inserts

   Sth1Cas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified; Integrative in mycobactreia

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid

    #161934

    Purpose

    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

    Depositor

    Christian Fankhauser

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. xCas9 3.7

    Plasmid

    #108379

    Purpose

    Mammalian expression vector for xCas9 3.7

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSG7-Wt-NmeCas9

    Plasmid

    #71474

    Purpose

    bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-WT-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW91.dCas9

    Plasmid

    #104372

    Purpose

    Expresses dCas9

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, S. Pyogenes, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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