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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Publication

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Publication

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Available since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pH2B-emiRFP670

Plasmid

#136571

Purpose

Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HA PKB T308D S473D pcDNA3

Plasmid

#14751

Depositor

Jim Woodgett

Publication

Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKB DD (AKT1 Bovine)

Tags

HA

Expression

Mammalian

Mutation

T308D S473D. Constitutively active mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

23 Articles

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

jun B oncogene (JUNB Human)

Tags

Flag

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Publication

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Publication

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Publication

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLentiPGK Blast DEST JNKKTRmRuby2

Plasmid

#59154

Purpose

Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Publication

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti-MP2

Plasmid

#36097

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Publication

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Type

Empty backbone

Use

Lentiviral

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAG-EGFP

Plasmid

#89684

Purpose

pCAG promoter driving EGFP

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Publication

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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