We narrowed to 39,127 results for: Eras

1. pThermeleon

   Plasmid

   #86120

   Purpose

   Bandpass temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   TcI38
   mCherry
   cI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

   Promoter

   pR/pL, pR/pL, pLacI, and pTlpA

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. hum sgk promoter F4R4

   Plasmid

   #83426

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

   Insert

   human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   sgk

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2ADW-4146NES(ROCK)

   Plasmid

   #162485

   Purpose

   For generating transgenic mice expressing ROCK biosensor based on FRET.

   Depositor

   Michiyuki Matsuda

   Article

   Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

   Insert

   ROCK biosensor based on FRET.
   

   Use

   Tags

   YPet and ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL-mCherry

   Plasmid

   #78862

   Purpose

   DULIP negative control (prey) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nanog5P reporter

   Plasmid

   #16337

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   Nanog promoter (Nanog Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Nanog promoter

   Available since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-S-Luc_cyto

   Plasmid

   #113926

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   S-Luc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLBac-FluB-trimer

   Plasmid

   #229859

   Purpose

   Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

   Depositor

   Stephen Cusack

   Article

   Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

   Inserts

   PA, Influenza B (B/Memphis/13/03)
   PB1, Influenza B (B/Memphis/13/03)
   PB2, Influenza B (B/Los Angeles/1/02)
   

   Use

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. JD631

   Plasmid

   #160399

   Purpose

   Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   35S::vitis GRF4-GIF1 chimera
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-HA-PARP11

   Plasmid

   #22556

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.R_TRE_minTK

    Plasmid

    #211516

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SV40 and minTK

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSplit2-NO-PRPF6

    Plasmid

    #51740

    Purpose

    Express LucN-PRPF6 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    PRP6 (PRPF6 Human)
    

    Use

    Tags

    FLAG and Luciferase (1-398aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTM1_VP35_ZEBOV

    Plasmid

    #68121

    Purpose

    Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP35 ZEBOV (VP35 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2-Basic-Myogenin core promoter

    Plasmid

    #134722

    Purpose

    Myogenin core promoter in a luciferase reporter plasmid

    Depositor

    Michael Chin

    Article

    Sun et al J Biol Chem. 2001 May 25;276(21):18591-6

    Insert

    Myogenin Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Myogenin

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TO-hNGN2-BSD-mApple

    Plasmid

    #182308

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    bsd-T2A-mycNLS-mApple
    

    Use

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4-p100

    Plasmid

    #23287

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    NFkB p100 subunit (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.27_GRHL-SPE

    Plasmid

    #177774

    Purpose

    Luciferase reporter for GRHL

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTM1_VP30_ZEBOV

    Plasmid

    #69119

    Purpose

    Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP30 ZEBOV (VP30 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mNFAT4-462:TALE:VP64:KRɸ

    Plasmid

    #160662

    Purpose

    Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    mNFAT4-462:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGL4.23_Mmp11

    Plasmid

    #209520

    Purpose

    Expresses luciferase under control of Mmp11 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

    Insert

    Mmp11 promoter (Mmp11 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVvanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLVX-RT002-LV

    Plasmid

    #163382

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEGFRtm (LV)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-RosaR4 KKR mutations

    Plasmid

    #37199

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to RosaR4 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs R4, and SV40 NLS

    Expression

    Mammalian

    Mutation

    I538K, E490K, and H537R

    Promoter

    CMV

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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26. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3 Flag mPTIP

    Plasmid

    #15557

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PTIP (Paxip1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pCMV4-Tax(M47)

    Plasmid

    #23286

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    Tax(M47)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L319R L320S

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pMOD_C'5802

    Plasmid

    #176879

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10:Luc+:RbcsE9
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198046

    Purpose

    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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