We narrowed to 5,829 results for: pcas

1. ePPE-V223A

   Plasmid

   #205243

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   SV40_NLS-nCas9-NC-MLV-SV40_NLS
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFPL-Gg

   Plasmid

   #61653

   Purpose

   Gateway vector for generating green fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced green fluorescent protein
   

   Expression

   Plant

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAYP (pDre)

   Plasmid

   #62709

   Purpose

   Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   Dre
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEP115E

   Plasmid

   #97401

   Purpose

   binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Tags

   Golgi target

   Expression

   Plant

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEP116E

   Plasmid

   #97402

   Purpose

   binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Tags

   Plasma membrane target

   Expression

   Plant

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_Blasto

   Plasmid

   #167856

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

   Plasmid

   #32474

   Purpose

   Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAMQ79G,Q139G-5HT3 HC
   

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTub-Wg

    Plasmid

    #64321

    Purpose

    Expresses Drosophila Wingless protein under the tubulin promotor

    Depositor

    Roel Nusse

    Article

    Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

    Insert

    Wingless (wg Fly)
    

    Use

    Drosophila expression

    Promoter

    alpha-1 tubulin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. p2T-CMV-AIDmax-BlastR

    Plasmid

    #152994

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    AID-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AL12R-HSP101

    Plasmid

    #161014

    Purpose

    PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC016-gPsp-EGFP_start_codon

    Plasmid

    #233613

    Purpose

    Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    gRNA targeting EGFP
    

    Use

    CRISPR

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CMV-ABEmaxCP1041-BlastR

    Plasmid

    #152990

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9 CP1041
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2T-CMV-BE4maxCP1028-BlastR

    Plasmid

    #152993

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4-nSpCas9 CP1028-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GB4080

    Plasmid

    #215249

    Purpose

    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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