We narrowed to 10,893 results for: ENA;

1. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs_FLAG-MCART2

   Plasmid

   #133250

   Purpose

   Express FLAG-tagged MCART2 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   MCART2 (SLC25A52 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Promoter

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

   Plasmid

   #61411

   Purpose

   Expresses human OTUD3 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 276-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_C2616

   Plasmid

   #91090

   Purpose

   Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   At7SL

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINK-OTUD5 (OTU, aa 171-358)

   Plasmid

   #61415

   Purpose

   Expresses human OTUD5 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD5 (OTUD5 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-170 and aa 359-571.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pASPIre1

   Plasmid

   #154842

   Purpose

   Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Prha

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs_FLAG-NDT1

   Plasmid

   #133254

   Purpose

   Express FLAG-tagged NDT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 (YIA6 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Promoter

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti CRISPR sgMTHFD2_1

   Plasmid

   #106313

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2 (MTHFD2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTriEx-mCherry-LINXa3

    Plasmid

    #73614

    Purpose

    Light-induced nuclear export switch for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Chicken B-Actin

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUW-TetO-Dppa2

    Plasmid

    #40799

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

    Insert

    Dppa2 (Dppa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV + TetO

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-hSyn-eGFP-SynaptoZip

    Plasmid

    #122525

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pASPIre3

    Plasmid

    #154844

    Purpose

    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_B2615

    Plasmid

    #91078

    Purpose

    Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N2-RIPK1 I539D

    Plasmid

    #78848

    Purpose

    To detect localization of RIPK1 I539D protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    I539D

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTRIP-SFFV-GFP-RELA K310R

    Plasmid

    #196630

    Purpose

    Express GFP-RELA K310R

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    GFP-RELA K310R (RELA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    K310R

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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