We narrowed to 24,925 results for: crispr
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Plasmid#136922PurposeExpresses ABEmax-NRTH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABEmax-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE GFP-Camk2a KI
Plasmid#131484PurposeEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SUV[SET]-dCas9
Plasmid#100088PurposeCatalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUVAR39H1 (SUV39H1 Human)
UseCRISPRTags3XFLag-NLS-SUV[SET]-dCas9-NLSExpressionMammalianMutationdeleted aa 1-76PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25H
Plasmid#91145PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSL
Plasmid#66590PurposeLox-STOP-Lox reporterDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSL-Tomato
Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9/VRQR-sgRNA (BbsI)
Plasmid#129725PurposeExpressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAMDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SaABEmax
Plasmid#119814PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA-ecTadA*(7.10)-nSaCas9
ExpressionMammalianAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_AncBE4max_3xHA
Plasmid#112097PurposeC:G-to-T:A base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAncBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11-mCherry
Plasmid#179682PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
ExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPED
Plasmid#162468PurposeDestination Expression vector for dicot plant prime editingDepositorInsertsCas9H840A
attR gateway destination cassette
TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to Alanine based sequence o…Promoter2x35SAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
enAsABE8e
Plasmid#138506PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA(8e)-dAsCas12a (En)
ExpressionMammalianAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJC6-GLS-3xFLAG
Plasmid#163450Purposelentiviral overexpression vector for human GLSDepositorInsertGLS (GLS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC007 - pRFP constitutive expression
Plasmid#79156PurposeRFP constitutively expressedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRFP
Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-sgCDKN1B#2 BFP
Plasmid#60905PurposeA lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFPDepositorInsertBFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by