We narrowed to 11,377 results for: ENA

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pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgROSA26

Plasmid

#231562

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgROSA26

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcU6_1 sgRNA

Plasmid

#92395

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.1 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.1

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2101

Plasmid

#91060

Purpose

Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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p3R112C/T180CBM3

Plasmid

#85090

Purpose

Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The R112C/T180C variant of PCNA3 fused to P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

Promoter

T7 promoter

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a-EGFP

Plasmid

#205311

Purpose

Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

Zebrafish expression

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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IDE-2Strep

Plasmid

#210355

Purpose

Expresses IDE fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

IDE (IDE Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-strepII-3C-LIC-strep

Plasmid

#108708

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170816

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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GLG1-EGFP

Plasmid

#210372

Purpose

Expresses GLG1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

GLG1 (GLG1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_HOIP/RNF31_#6

Plasmid

#75252

Purpose

retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against human RNF31 (RNF31 )

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pEY43

Plasmid

#191047

Purpose

eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

eat-4(prom6-1) (eat-4 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I546K-peGFP-N1

Plasmid

#228582

Purpose

Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I546K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6A (full-length, aa 1-288)

Plasmid

#61416

Purpose

Expresses human OTUD6A (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_B2103b

Plasmid

#91062

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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