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pAAV-anti-VEGF(Nb35) PAGER(FL)

Plasmid

#230025

Purpose

Expresses anti-VEGF PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Nb35-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gs)

Plasmid

#230007

Purpose

Expresses anti-GFP PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1Dxs

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(G12)

Plasmid

#230009

Purpose

Expresses anti-GFP PAGER(G12) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1Dx12

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG/Luci

Plasmid

#139981

Purpose

Expresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Luciferase

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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Puro-iDEST

Plasmid

#75336

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

Plasmid

#171432

Purpose

Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-hH3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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LentiUniversal-Puro

Plasmid

#127749

Purpose

Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

Depositor

Giulio Draetta

Article

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pV1F

Plasmid

#205084

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Expression

Bacterial

Promoter

agorbacterium virB operon promoter

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lentiviral-FKBP-cpGFP-Nb39

Plasmid

#191498

Purpose

Rapamycin-dependent opioid sensor for opioid receptors (component 2)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

FKBP-cpGFP-Nb39

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFosill-4

Plasmid

#89698

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVSP6-NR2F2-SV40-PURO

Plasmid

#138361

Purpose

Lentiviral plasmid contains human NR2F2 driven by CMVSP6 and puromycin resistance for selection

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Human NR2F2, with puromycin driven by SV40 for selection

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Cas12j2

Plasmid

#188498

Purpose

Expresses FLAG tagged Cas12j2

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti‐puro‐OgRNA_mdxE53

Plasmid

#187054

Purpose

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-PBE

Plasmid

#119772

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

NLS-APOBEC1-nCas9-UGI-NLS

Tags

NLS

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ150-set2

Plasmid

#82546

Purpose

Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::set-2 3'UTR

Expression

Worm

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-proCaspase-3-TEVp

Plasmid

#166607

Purpose

Encodes Cre-dependent procaspase 3 and TEVp under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

procaspase3- TEVp

Use

AAV

Promoter

tetracycline response element

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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